Home / IFN-beta / Mouse IFN-beta DuoSet ELISA

Mouse IFN-beta DuoSet ELISA

Catalog #: DY8234-05
38 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY8234-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Mouse IFN-beta ELISA Standard Curve
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Product Details
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Citations (38)
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Reviews (6)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IFN-beta DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Range
12.5 - 800 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Interferon beta (IFN-β). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse IFN-beta ELISA Standard Curve View Larger

Mouse IFN-beta ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name:
Interferon beta
Entrez Gene IDs:
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names:
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IFN-beta DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. H7N7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitro
    Authors: Gabele, L;Bochow, I;Rieke, N;Sieben, C;Michaelsen-Preusse, K;Hosseini, S;Korte, M;
    Frontiers in cellular neuroscience
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Intratumoral NKT cell accumulation promotes antitumor immunity in pancreatic cancer
    Authors: Li, J;Moresco, P;Fearon, DT;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Rujin Jiedu decoction protects against influenza virus infection by modulating gut microbiota
    Authors: Huang, Q;Yang, G;Tang, C;Dou, B;Hu, Y;Liu, H;Wu, X;Zhang, H;Wang, H;Xu, L;Yang, XD;Xu, Y;Zheng, Y;
    Heliyon
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
    Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates, BALF
  5. TLR9 Monotherapy in Immune-Competent Mice Suppresses Orthotopic Prostate Tumor Development
    Authors: Miles, MA;Luong, R;To, EE;Erlich, JR;Liong, S;Liong, F;Logan, JM;O'Leary, J;Brooks, DA;Selemidis, S;
    Cells
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Protein Concentration Affects the Food Allergen ?-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells
    Authors: Heinzl, GC;Eriksen, DB;Johnsen, PR;Scarafoni, A;Frøkiær, H;
    Biomolecules
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation
    Authors: Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J;
    Nature communications
    Species: Mouse
    Sample Types: Serum
  8. Development of LB244, an Irreversible STING Antagonist
    Authors: Barasa, L;Chaudhuri, S;Zhou, JY;Jiang, Z;Choudhary, S;Green, RM;Wiggin, E;Cameron, M;Humphries, F;Fitzgerald, KA;Thompson, PR;
    Journal of the American Chemical Society
    Species: Mouse
    Sample Types: Cell Culture Supernates, Serum
  9. Loss of PARP7 Increases Type I Interferon Signaling in EO771 Breast Cancer Cells and Prevents Mammary Tumor Growth by Increasing Antitumor Immunity
    Authors: Rasmussen, M;Alvik, K;Kannen, V;Olafsen, NE;Erlingsson, LAM;Grimaldi, G;Takaoka, A;Grant, DM;Matthews, J;
    Cancers
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS
    Authors: Pérez-Cabello, JA;Silvera-Carrasco, L;Franco, JM;Capilla-González, V;Armaos, A;Gómez-Lima, M;García-García, R;Yap, XW;Leal-Lasarte, M;Lall, D;Baloh, RH;Martínez, S;Miyata, Y;Tartaglia, GG;Sawarkar, R;García-Domínguez, M;Pozo, D;Roodveldt, C;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. VANGL2 inhibits antiviral IFN-I signaling by targeting TBK1 for autophagic degradation
    Authors: Hu, Z;Xie, Y;Lu, J;Yang, J;Zhang, J;Jiang, H;Li, H;Zhang, Y;Wu, D;Zeng, K;Bai, X;Yu, X;
    Science advances
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates, Serum
  12. Loss of Gasdermin D is protective against influenza virus-induced inflammation and mortality
    Authors: S Speaks, A Zani, A Solstad, A Kenney, L Zhang, AC Eddy, AO Amer, R Robinson, C Cai, J Ma, EA Hemann, A Forero, JS Yount
    bioRxiv : the preprint server for biology, 2023-04-07;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism
    Authors: D Levy, SN Abadchi, N Shababi, MR Ravari, NH Pirolli, C Bergeron, A Obiorah, F Mokhtari-E, S Gheshlaghi, JM Abraham, IM Smith, E Powsner, T Solomon, JW Harmon, SM Jay
    bioRxiv : the preprint server for biology, 2023-03-23;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Epigenetic state determines the in vivo efficacy of STING agonist therapy
    Authors: R Falahat, A Berglund, P Perez-Vill, RM Putney, I Hamaidi, S Kim, S Pilon-Thom, GN Barber, JJ Mulé
    Nature Communications, 2023-03-22;14(1):1573.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
    Authors: A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill
    Nature, 2023-03-08;615(7952):490-498.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates
  16. Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages
    Authors: Y Wang, Y Wang, L Ding, X Ren, B Wang, L Wang, S Zhao, X Yue, Z Wu, C Li, X Liang, C Ma, L Gao
    Cell Reports, 2022-11-29;41(9):111738.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates
  17. DOT1L regulates lipid biosynthesis and inflammatory responses in macrophages and promotes atherosclerotic plaque stability
    Authors: L Willemsen, KHM Prange, AE Neele, CPAA van Roomen, M Gijbels, GR Griffith, MD Toom, L Beckers, R Siebeler, NJ Spann, HJ Chen, LA Bosmans, A Gorbatenko, S van Wouw, N Zelcer, H Jacobs, F van Leeuwe, MPJ de Winther
    Cell Reports, 2022-11-22;41(8):111703.
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates
  18. The retroelement Lx9 puts a brake on the immune response to virus infection
    Authors: N Bartonicek, R Rouet, J Warren, C Loetsch, GS Rodriguez, S Walters, F Lin, D Zahra, J Blackburn, JM Hammond, ALM Reis, IW Deveson, N Zammit, M Zeraati, S Grey, D Christ, JS Mattick, T Chtanova, R Brink, ME Dinger, RJ Weatheritt, J Sprent, C King
    Nature, 2022-08-10;0(0):.
    Species: Mouse
    Sample Types: Serum
  19. Deactylation by SIRT1 enables liquid-liquid phase separation of IRF3/IRF7 in innate antiviral immunity
    Authors: Z Qin, X Fang, W Sun, Z Ma, T Dai, S Wang, Z Zong, H Huang, H Ru, H Lu, B Yang, S Lin, F Zhou, L Zhang
    Nature Immunology, 2022-07-25;0(0):.
    Species: Mouse
    Sample Types: Serum
  20. Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns
    Authors: M Perego, S Fu, Y Cao, A Kossenkov, M Yao, K Alicea-Tor, W Liu, Z Jiang, Z Chen, SY Fuchs, J Zhou, DI Gabrilovic
    Oncogene, 2022-06-21;0(0):.
    Species: Mouse
    Sample Types: Plasma
  21. Multiplexed electrospray enables high throughput production of cGAMP microparticles to serve as an adjuvant for a broadly acting influenza vaccine
    Authors: CJ Batty, MD Gallovic, J Williams, TM Ross, EM Bachelder, KM Ainslie
    International journal of pharmaceutics, 2022-05-24;622(0):121839.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Influenza virus replication in cardiomyocytes drives heart dysfunction and fibrosis
    Authors: AD Kenney, SL Aron, C Gilbert, N Kumar, P Chen, A Eddy, L Zhang, A Zani, N Vargas-Mal, S Speaks, J Kawahara, PJ Denz, L Dorn, F Accornero, J Ma, H Zhu, MVS Rajaram, C Cai, RA Langlois, JS Yount
    Science Advances, 2022-05-11;8(19):eabm5371.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. STING mediates neurodegeneration and neuroinflammation in nigrostriatal alpha-synucleinopathy
    Authors: JT Hinkle, J Patel, N Panicker, SS Karuppagou, D Biswas, B Belingon, R Chen, S Brahmachar, O Pletnikova, JC Troncoso, VL Dawson, TM Dawson
    Proceedings of the National Academy of Sciences of the United States of America, 2022-04-08;119(15):e2118819119.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics
    Authors: D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas
    Embo Molecular Medicine, 2021-11-11;0(0):e12924.
    Species: Mouse
    Sample Types: Plasma
  25. Enhancing adoptive CD8 T cell therapy by systemic delivery of tumor associated antigens
    Authors: DE Jæhger, ML Hübbe, MK Kræmer, G Clergeaud, AV Olsen, C Stavnsbjer, MN Wiinholt, A Kjær, JR Henriksen, AE Hansen, TL Andresen
    Scientific Reports, 2021-10-05;11(1):19794.
    Species: Mouse
    Sample Types: Serum
  26. Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF
    Authors: S Rastogi, S Ellinwood, J Augenstrei, KD Mayer-Barb, V Briken
    PloS Pathogens, 2021-07-29;17(7):e1009712.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis
    Authors: Y Li, C Bax, J Patel, T Vazquez, A Ravishanka, MM Bashir, M Grinnell, D Diaz, VP Werth
    Theranostics, 2021-05-21;11(15):7144-7158.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. In situ cancer vaccination using lipidoid nanoparticles
    Authors: J Chen, M Qiu, Z Ye, T Nyalile, Y Li, Z Glass, X Zhao, L Yang, J Chen, Q Xu
    Science Advances, 2021-05-05;7(19):.
    Species: Mouse
    Sample Types: Serum
  29. A NOVEL NOX/PHOX-CD38-NAADP-TFEB AXIS IMPORTANT FOR MACROPHAGE ACTIVATION DURING BACTERIAL PHAGOCYTOSIS
    Authors: M Najibi, HH Honwad, JA Moreau, SM Becker, JE Irazoqui
    Autophagy, 2021-04-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors
    Authors: N Hijaze, M Ledersnaid, E Simanovich, S Kassem, MA Rahat
    J Leukoc Biol, 2020-11-17;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis
    Authors: JC DuBois, AK Ray, P Davies, B Shafit-Zag
    J Neuroinflammation, 2020-10-29;17(1):324.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling
    Authors: M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma
    Nat Commun, 2020-07-17;11(1):3624.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Skeletal muscle antagonizes antiviral CD8+ T cell exhaustion
    Authors: J Wu, N Weisshaar, A Hotz-Wagen, A Madi, S Ma, A Mieg, M Hering, K Mohr, T Schlimbach, H Borgers, G Cui
    Sci Adv, 2020-06-12;6(24):eaba3458.
    Species: Mouse
    Sample Types: Serum
  34. Rupintrivir reduces RV-induced TH-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo
    Authors: O Danov, L Lasswitz, H Obernolte, C Hesse, A Braun, S Wronski, K Sewald
    Respir. Res., 2019-10-22;20(1):228.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. PTP1B negatively regulates nitric oxide-mediated Pseudomonas aeruginosa killing by neutrophils
    Authors: L Yue, M Yan, ML Tremblay, TJ Lin, H Li, T Yang, X Song, T Xie, Z Xie
    PLoS ONE, 2019-09-18;14(9):e0222753.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. Cathelicidins Inhibit Escherichia coli-Induced TLR2 and TLR4 Activation in a Viability-Dependent Manner
    Authors: M Coorens, VAF Schneider, AM de Groot, A van Dijk, M Meijerink, JM Wells, MR Scheenstra, EJA Veldhuizen, HP Haagsman
    J. Immunol., 2017-07-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. TLR7 and TLR3 Sense Brucella abortus RNA to Induce Proinflammatory Cytokine Production but They Are Dispensable for Host Control of Infection
    Authors: PC Campos, MT Gomes, ES Guimarães, G Guimarães, SC Oliveira
    Front Immunol, 2017-01-23;8(0):28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection
    Authors: Joerg Mattes
    Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Reviews for Mouse IFN-beta DuoSet ELISA

Average Rating: 5 (Based on 6 Reviews)

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Mouse IFN-beta DuoSet ELISA
By Anonymous on 06/23/2023
Sample Tested: 293T human embryonic kidney cell line,HEK293 cell line
Mouse IFN-beta DuoSet ELISA
By Anonymous on 12/14/2020
Sample Tested: Mouse lymphocytes
Mouse IFN-beta DuoSet ELISA DY8234-05
Mouse IFN-beta DuoSet ELISA
By Anonymous on 12/12/2019
Sample Tested: mouse microglia
Mouse IFN-beta DuoSet ELISA DY8234-05
Mouse IFN-beta DuoSet ELISA
By Anonymous on 09/11/2019
Sample Tested: Mouse spleenocytes
Mouse IFN-beta DuoSet ELISA
By Kim Han on 01/03/2019
Sample Tested: Cell culture supernatant
Mouse IFN-beta DuoSet ELISA
By Anonymous on 09/25/2018
Sample Tested: Mouse,Spinal cord tissue
Mouse IFN-beta DuoSet ELISA DY8234-05