Mouse IFN-beta DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: IFN-beta
Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Mouse IFN-beta DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Innate immune control of influenza virus interspecies adaptation via IFITM3
Authors: Denz, PJ;Speaks, S;Kenney, AD;Eddy, AC;Papa, JL;Roettger, J;Scace, SC;Rubrum, A;Hemann, EA;Forero, A;Webby, RJ;Bowman, AS;Yount, JS;
Nature communications
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates
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Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution
Authors: Hong, NE;Chaplin, A;Di, L;Ravodina, A;Bevan, GH;Gao, H;Asase, C;Gangwar, RS;Cameron, MJ;Mignery, M;Cherepanova, O;Finn, AV;Nayak, L;Pieper, AA;Maiseyeu, A;
Cell reports
Species: Mouse
Sample Types: Cell Culture Supernates
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The potential of mesenchymal stem cell coexpressing cytosine deaminase and secretory IL18-FC chimeric cytokine in suppressing glioblastoma recurrence
Authors: Taheri, M;Tehrani, HA;Farzad, SA;Korourian, A;Arefian, E;Ramezani, M;
International immunopharmacology
Species: Mouse
Sample Types: Serum
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An in situ depot for the sustained release of a TLR7/8 agonist in combination with a TGF? inhibitor promotes anti-tumor immune responses
Authors: Jensen, SB;Jæhger, DE;Serrano-Chávez, E;Halldórsdóttir, HR;Engel, TB;Jørgensen, JS;Björgvinsdóttir, UJ;Kostrikov, S;Scheeper, MJ;Ringgaard, L;Bruun, LM;Stavnsbjerg, C;Christensen, E;Bak, M;Thuroczy, J;Balogh, L;Jensen, ATI;Melander, F;Kjaer, A;Henriksen, JR;Hansen, AE;Andresen, TL;
Nature communications
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates
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H7N7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitro
Authors: Gabele, L;Bochow, I;Rieke, N;Sieben, C;Michaelsen-Preusse, K;Hosseini, S;Korte, M;
Frontiers in cellular neuroscience
Species: Mouse
Sample Types: Cell Culture Supernates
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Intratumoral NKT cell accumulation promotes antitumor immunity in pancreatic cancer
Authors: Li, J;Moresco, P;Fearon, DT;
Proceedings of the National Academy of Sciences of the United States of America
Species: Mouse
Sample Types: Cell Culture Supernates
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Rujin Jiedu decoction protects against influenza virus infection by modulating gut microbiota
Authors: Huang, Q;Yang, G;Tang, C;Dou, B;Hu, Y;Liu, H;Wu, X;Zhang, H;Wang, H;Xu, L;Yang, XD;Xu, Y;Zheng, Y;
Heliyon
Species: Mouse
Sample Types: Cell Culture Supernates
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Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
Nature communications
Species: Mouse, Transgenic Mouse
Sample Types: Cell Culture Supernates, BALF
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TLR9 Monotherapy in Immune-Competent Mice Suppresses Orthotopic Prostate Tumor Development
Authors: Miles, MA;Luong, R;To, EE;Erlich, JR;Liong, S;Liong, F;Logan, JM;O'Leary, J;Brooks, DA;Selemidis, S;
Cells
Species: Mouse
Sample Types: Cell Culture Supernates
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Protein Concentration Affects the Food Allergen ?-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells
Authors: Heinzl, GC;Eriksen, DB;Johnsen, PR;Scarafoni, A;Frøkiær, H;
Biomolecules
Species: Mouse
Sample Types: Cell Culture Supernates
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Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation
Authors: Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J;
Nature communications
Species: Mouse
Sample Types: Serum
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Development of LB244, an Irreversible STING Antagonist
Authors: Barasa, L;Chaudhuri, S;Zhou, JY;Jiang, Z;Choudhary, S;Green, RM;Wiggin, E;Cameron, M;Humphries, F;Fitzgerald, KA;Thompson, PR;
Journal of the American Chemical Society
Species: Mouse
Sample Types: Cell Culture Supernates, Serum
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Loss of PARP7 Increases Type I Interferon Signaling in EO771 Breast Cancer Cells and Prevents Mammary Tumor Growth by Increasing Antitumor Immunity
Authors: Rasmussen, M;Alvik, K;Kannen, V;Olafsen, NE;Erlingsson, LAM;Grimaldi, G;Takaoka, A;Grant, DM;Matthews, J;
Cancers
Species: Mouse
Sample Types: Cell Culture Supernates
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MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS
Authors: Pérez-Cabello, JA;Silvera-Carrasco, L;Franco, JM;Capilla-González, V;Armaos, A;Gómez-Lima, M;García-García, R;Yap, XW;Leal-Lasarte, M;Lall, D;Baloh, RH;Martínez, S;Miyata, Y;Tartaglia, GG;Sawarkar, R;García-Domínguez, M;Pozo, D;Roodveldt, C;
Proceedings of the National Academy of Sciences of the United States of America
Species: Mouse
Sample Types: Cell Culture Supernates
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VANGL2 inhibits antiviral IFN-I signaling by targeting TBK1 for autophagic degradation
Authors: Hu, Z;Xie, Y;Lu, J;Yang, J;Zhang, J;Jiang, H;Li, H;Zhang, Y;Wu, D;Zeng, K;Bai, X;Yu, X;
Science advances
Species: Transgenic Mouse
Sample Types: Cell Culture Supernates, Serum
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Loss of Gasdermin D is protective against influenza virus-induced inflammation and mortality
Authors: S Speaks, A Zani, A Solstad, A Kenney, L Zhang, AC Eddy, AO Amer, R Robinson, C Cai, J Ma, EA Hemann, A Forero, JS Yount
bioRxiv : the preprint server for biology, 2023-04-07;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism
Authors: D Levy, SN Abadchi, N Shababi, MR Ravari, NH Pirolli, C Bergeron, A Obiorah, F Mokhtari-E, S Gheshlaghi, JM Abraham, IM Smith, E Powsner, T Solomon, JW Harmon, SM Jay
bioRxiv : the preprint server for biology, 2023-03-23;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Epigenetic state determines the in vivo efficacy of STING agonist therapy
Authors: R Falahat, A Berglund, P Perez-Vill, RM Putney, I Hamaidi, S Kim, S Pilon-Thom, GN Barber, JJ Mulé
Nature Communications, 2023-03-22;14(1):1573.
Species: Mouse
Sample Types: Cell Culture Supernates
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Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
Authors: A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill
Nature, 2023-03-08;615(7952):490-498.
Species: Mouse, Transgenic Mouse
Sample Types: Cell Culture Supernates
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Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages
Authors: Y Wang, Y Wang, L Ding, X Ren, B Wang, L Wang, S Zhao, X Yue, Z Wu, C Li, X Liang, C Ma, L Gao
Cell Reports, 2022-11-29;41(9):111738.
Species: Mouse, Transgenic Mouse
Sample Types: Cell Culture Supernates
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DOT1L regulates lipid biosynthesis and inflammatory responses in macrophages and promotes atherosclerotic plaque stability
Authors: L Willemsen, KHM Prange, AE Neele, CPAA van Roomen, M Gijbels, GR Griffith, MD Toom, L Beckers, R Siebeler, NJ Spann, HJ Chen, LA Bosmans, A Gorbatenko, S van Wouw, N Zelcer, H Jacobs, F van Leeuwe, MPJ de Winther
Cell Reports, 2022-11-22;41(8):111703.
Species: Transgenic Mouse
Sample Types: Cell Culture Supernates
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The retroelement Lx9 puts a brake on the immune response to virus infection
Authors: N Bartonicek, R Rouet, J Warren, C Loetsch, GS Rodriguez, S Walters, F Lin, D Zahra, J Blackburn, JM Hammond, ALM Reis, IW Deveson, N Zammit, M Zeraati, S Grey, D Christ, JS Mattick, T Chtanova, R Brink, ME Dinger, RJ Weatheritt, J Sprent, C King
Nature, 2022-08-10;0(0):.
Species: Mouse
Sample Types: Serum
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Deactylation by SIRT1 enables liquid-liquid phase separation of IRF3/IRF7 in innate antiviral immunity
Authors: Z Qin, X Fang, W Sun, Z Ma, T Dai, S Wang, Z Zong, H Huang, H Ru, H Lu, B Yang, S Lin, F Zhou, L Zhang
Nature Immunology, 2022-07-25;0(0):.
Species: Mouse
Sample Types: Serum
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Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns
Authors: M Perego, S Fu, Y Cao, A Kossenkov, M Yao, K Alicea-Tor, W Liu, Z Jiang, Z Chen, SY Fuchs, J Zhou, DI Gabrilovic
Oncogene, 2022-06-21;0(0):.
Species: Mouse
Sample Types: Plasma
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Multiplexed electrospray enables high throughput production of cGAMP microparticles to serve as an adjuvant for a broadly acting influenza vaccine
Authors: CJ Batty, MD Gallovic, J Williams, TM Ross, EM Bachelder, KM Ainslie
International journal of pharmaceutics, 2022-05-24;622(0):121839.
Species: Mouse
Sample Types: Cell Culture Supernates
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Influenza virus replication in cardiomyocytes drives heart dysfunction and fibrosis
Authors: AD Kenney, SL Aron, C Gilbert, N Kumar, P Chen, A Eddy, L Zhang, A Zani, N Vargas-Mal, S Speaks, J Kawahara, PJ Denz, L Dorn, F Accornero, J Ma, H Zhu, MVS Rajaram, C Cai, RA Langlois, JS Yount
Science Advances, 2022-05-11;8(19):eabm5371.
Species: Mouse
Sample Types: Tissue Homogenates
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STING mediates neurodegeneration and neuroinflammation in nigrostriatal alpha-synucleinopathy
Authors: JT Hinkle, J Patel, N Panicker, SS Karuppagou, D Biswas, B Belingon, R Chen, S Brahmachar, O Pletnikova, JC Troncoso, VL Dawson, TM Dawson
Proceedings of the National Academy of Sciences of the United States of America, 2022-04-08;119(15):e2118819119.
Species: Mouse
Sample Types: Cell Culture Supernates
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Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics
Authors: D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas
Embo Molecular Medicine, 2021-11-11;0(0):e12924.
Species: Mouse
Sample Types: Plasma
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Enhancing adoptive CD8 T cell therapy by systemic delivery of tumor associated antigens
Authors: DE Jæhger, ML Hübbe, MK Kræmer, G Clergeaud, AV Olsen, C Stavnsbjer, MN Wiinholt, A Kjær, JR Henriksen, AE Hansen, TL Andresen
Scientific Reports, 2021-10-05;11(1):19794.
Species: Mouse
Sample Types: Serum
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Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF
Authors: S Rastogi, S Ellinwood, J Augenstrei, KD Mayer-Barb, V Briken
PloS Pathogens, 2021-07-29;17(7):e1009712.
Species: Mouse
Sample Types: Cell Culture Supernates
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Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis
Authors: Y Li, C Bax, J Patel, T Vazquez, A Ravishanka, MM Bashir, M Grinnell, D Diaz, VP Werth
Theranostics, 2021-05-21;11(15):7144-7158.
Species: Mouse
Sample Types: Cell Culture Supernates
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In situ cancer vaccination using lipidoid nanoparticles
Authors: J Chen, M Qiu, Z Ye, T Nyalile, Y Li, Z Glass, X Zhao, L Yang, J Chen, Q Xu
Science Advances, 2021-05-05;7(19):.
Species: Mouse
Sample Types: Serum
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A NOVEL NOX/PHOX-CD38-NAADP-TFEB AXIS IMPORTANT FOR MACROPHAGE ACTIVATION DURING BACTERIAL PHAGOCYTOSIS
Authors: M Najibi, HH Honwad, JA Moreau, SM Becker, JE Irazoqui
Autophagy, 2021-04-13;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors
Authors: N Hijaze, M Ledersnaid, E Simanovich, S Kassem, MA Rahat
J Leukoc Biol, 2020-11-17;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis
Authors: JC DuBois, AK Ray, P Davies, B Shafit-Zag
J Neuroinflammation, 2020-10-29;17(1):324.
Species: Mouse
Sample Types: Cell Culture Supernates
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MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling
Authors: M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma
Nat Commun, 2020-07-17;11(1):3624.
Species: Human
Sample Types: Cell Culture Supernates
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Skeletal muscle antagonizes antiviral CD8+ T cell exhaustion
Authors: J Wu, N Weisshaar, A Hotz-Wagen, A Madi, S Ma, A Mieg, M Hering, K Mohr, T Schlimbach, H Borgers, G Cui
Sci Adv, 2020-06-12;6(24):eaba3458.
Species: Mouse
Sample Types: Serum
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Rupintrivir reduces RV-induced TH-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo
Authors: O Danov, L Lasswitz, H Obernolte, C Hesse, A Braun, S Wronski, K Sewald
Respir. Res., 2019-10-22;20(1):228.
Species: Mouse
Sample Types: Cell Culture Supernates
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PTP1B negatively regulates nitric oxide-mediated Pseudomonas aeruginosa killing by neutrophils
Authors: L Yue, M Yan, ML Tremblay, TJ Lin, H Li, T Yang, X Song, T Xie, Z Xie
PLoS ONE, 2019-09-18;14(9):e0222753.
Species: Mouse
Sample Types: Cell Culture Supernates
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Cathelicidins Inhibit Escherichia coli-Induced TLR2 and TLR4 Activation in a Viability-Dependent Manner
Authors: M Coorens, VAF Schneider, AM de Groot, A van Dijk, M Meijerink, JM Wells, MR Scheenstra, EJA Veldhuizen, HP Haagsman
J. Immunol., 2017-07-14;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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TLR7 and TLR3 Sense Brucella abortus RNA to Induce Proinflammatory Cytokine Production but They Are Dispensable for Host Control of Infection
Authors: PC Campos, MT Gomes, ES Guimarães, G Guimarães, SC Oliveira
Front Immunol, 2017-01-23;8(0):28.
Species: Mouse
Sample Types: Cell Culture Supernates
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TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection
Authors: Joerg Mattes
Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200.
Species: Mouse
Sample Types: Tissue Homogenates
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