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Mouse IFN-beta ELISA Kit - Quantikine

Catalog #: MIFNB0
22 Citations 6 Reviews Datasheet / COA / SDS
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Mouse IFN-beta ELISA Standard Curve
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Citations (22)
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Reviews (6)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IFN-beta Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
1.89 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Ten serum samples, 5 EDTA plasma, and 5 heparin plasma samples were evaluated for the presence of mouse IFN-beta in this assay. No detectable levels were observed.

Cell Culture Supernates - - NIH-3T3 mouse embyronic fibroblast cells were cultured in DMEM supplemented with 10% fetal bovine serum and grown to approximately 90% confluency. Cells were unstimulated or stimulated with 10 μg/mL Poly I:C delivered via Lipofectamine for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of mouse IFN-beta.

Condition(pg/mL)
UnstimulatedND
Stimulated1768
ND=Non-detectable

Product Summary

The Quantikine® Mouse IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-beta in cell culture supernates, serum, and plasma. It contains
HEK293-expressed recombinant mouse IFN-beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IFN-beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.5 180 437 52.6 173 441
Standard Deviation 180 5.84 13.7 5.28 10 27.2
CV% 437 3.2 3.1 10 5.8 6.2

Recovery

The recovery of mouse IFN-beta spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 88-106
EDTA Plasma (n=4) 99 89-106
Heparin Plasma (n=4) 94 83-100
Serum (n=4) 94 89-97

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse IFN-beta ELISA Linearity

Scientific Data

N/A Mouse IFN-beta ELISA Standard Curve View Larger

Mouse IFN-beta ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name:
Interferon beta
Entrez Gene IDs:
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names:
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IFN-beta Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Microglial type I interferon signaling mediates chronic stress-induced synapse loss and social behavior deficits
    Authors: Tripathi, A;Bartosh, A;Mata, J;Jacks, C;Madeshiya, AK;Hussein, U;Hong, LE;Zhao, Z;Pillai, A;
    Molecular psychiatry
    Species: Mouse
    Sample Types: Serum
  2. An intranasal nanoparticle STING agonist protects against respiratory viruses in animal models
    Authors: Leekha, A;Saeedi, A;Kumar, M;Sefat, KMSR;Martinez-Paniagua, M;Meng, H;Fathi, M;Kulkarni, R;Reichel, K;Biswas, S;Tsitoura, D;Liu, X;Cooper, LJN;Sands, CM;Das, VE;Sebastian, M;Hurst, BL;Varadarajan, N;
    Nature communications
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Yeast ?-glucan promotes antiviral type I interferon response via dectin-1
    Authors: Wang, J;Jin, X;Yan, S;Zhao, H;Pang, D;Ouyang, H;Tang, X;
    Veterinary microbiology
    Species: Mouse
    Sample Types: Serum, Cell Culture Supernates
  4. An interferon-integrated mucosal vaccine provides pan-sarbecovirus protection in small animal models
    Authors: Yuen, CK;Wong, WM;Mak, LF;Lam, JY;Cheung, LY;Cheung, DT;Ng, YY;Lee, AC;Zhong, N;Yuen, KY;Kok, KH;
    Nature communications
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Mannose-Coated Reconstituted Lipoprotein Nanoparticles for the Targeting of Tumor-Associated Macrophages: Optimization, Characterization, and In Vitro Evaluation of Effectiveness
    Authors: Dossou, AS;Mantsch, ME;Kapic, A;Burnett, WL;Sabnis, N;Coffer, JL;Berg, RE;Fudala, R;Lacko, AG;
    Pharmaceutics
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
    Authors: A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill
    Nature, 2023-03-08;615(7952):490-498.
    Species: Mouse
    Sample Types: Serum
  7. Nonsteroidal anti-inflammatory drugs (NSAIDs) and nucleotide analog GS-441524 conjugates with potent in vivo efficacy against coronaviruses
    Authors: Q Zhou, Y Luo, Y Zhu, Q Chen, J Qiu, F Cong, Y Li, X Zhang
    European Journal of Medicinal Chemistry, 2023-01-10;249(0):115113.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Clostridium butyricum-induced ?-3 fatty acid 18-HEPE elicits anti-influenza virus pneumonia effects through interferon-lambda upregulation
    Authors: M Hagihara, M Yamashita, T Ariyoshi, S Eguchi, A Minemura, D Miura, S Higashi, K Oka, T Nonogaki, T Mori, K Iwasaki, J Hirai, Y Shibata, T Umemura, H Kato, N Asai, Y Yamagishi, A Ota, M Takahashi, H Mikamo
    Cell Reports, 2022-12-13;41(11):111755.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. 2'-5' oligoadenylate synthetase?like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability
    Authors: TK Kim, S Jeon, S Park, SK Sonn, S Seo, J Suh, J Jin, HY Kweon, S Kim, SH Moon, O Kweon, BH Koo, N Kim, HO Lee, YM Kim, YJ Kim, SH Park, GT Oh
    Nature Communications, 2022-11-04;13(1):6647.
    Species: Mouse
    Sample Types: Plasma
  10. Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity
    Authors: M Tomokiyo, FR Tonetti, H Yamamuro, R Shibata, K Fukuyama, N Gobbato, L Albarracin, MSR Rajoka, AKMH Kober, W Ikeda-Ohts, J Villena, H Kitazawa
    Cells, 2022-09-25;11(19):.
    Species: Mouse
    Sample Types: BALF
  11. USP25 deficiency exacerbates acute pancreatitis via upregulating TBK1-NF-kappaB signaling in macrophages
    Authors: X Liu, W Luo, J Chen, C Hu, RN Mutsinze, X Wang, Y Zhang, L Huang, W Zuo, G Liang, Y Wang
    Cellular and Molecular Gastroenterology and Hepatology, 2022-08-04;0(0):.
    Species: Mouse
    Sample Types: Serum
  12. SHR1032, a novel STING agonist, stimulates anti-tumor immunity and directly induces AML apoptosis
    Authors: C Song, D Liu, S Liu, D Li, I Horecny, X Zhang, P Li, L Chen, M Miller, R Chowdhury, M Issa, R Shen, Y Yan, F Zhang, L Zhang, L Zhang, C Bai, J Feng, L Zhuang, R Zhang, J Li, H Wilkinson, J Liu, W Tao
    Scientific Reports, 2022-05-20;12(1):8579.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. cGAS exacerbates Schistosoma japonicum infection in a STING-type I IFN-dependent and independent manner
    Authors: L Liang, Y Shen, Y Hu, H Liu, J Cao
    PloS Pathogens, 2022-02-02;18(2):e1010233.
    Species: Mouse
    Sample Types: Serum
  14. A natural product, (S)-10-Hydroxycamptothecin inhibits pseudorabies virus proliferation through DNA damage dependent antiviral innate immunity
    Authors: X Liu, L Lv, C Jiang, J Bai, Y Gao, Z Ma, P Jiang
    Veterinary Microbiology, 2021-12-20;265(0):109313.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. MAVS is energized by Mff which senses mitochondrial metabolism via AMPK for acute antiviral immunity
    Authors: Y Hanada, N Ishihara, L Wang, H Otera, T Ishihara, T Koshiba, K Mihara, Y Ogawa, M Nomura
    Nat Commun, 2020-11-11;11(1):5711.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Nrf2 mitigates prolonged PM2.5 exposure-triggered liver inflammation by positively regulating SIKE activity: Protection by Juglanin
    Authors: C Ge, J Tan, S Zhong, L Lai, G Chen, J Zhao, C Yi, L Wang, L Zhou, T Tang, Q Yang, D Lou, Q Li, Y Wu, L Hu, G Kuang, X Liu, B Wang, M Xu
    Redox Biol, 2020-07-17;36(0):101645.
    Species: Mouse
    Sample Types: Serum
  17. Xenogenization of tumor cells by fusogenic exosomes in tumor microenvironment ignites and propagates antitumor immunity
    Authors: GB Kim, GH Nam, Y Hong, J Woo, Y Cho, IC Kwon, Y Yang, IS Kim
    Science Advances, 2020-07-01;6(27):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Transient microglial absence assists postmigratory cortical neurons in proper differentiation
    Authors: Y Hattori, Y Naito, Y Tsugawa, S Nonaka, H Wake, T Nagasawa, A Kawaguchi, T Miyata
    Nat Commun, 2020-04-02;11(1):1631.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. A chemically unmodified agonistic DNA with growth factor functionality for in vivo therapeutic application
    Authors: R Ueki, S Uchida, N Kanda, N Yamada, A Ueki, M Akiyama, K Toh, H Cabral, S Sando
    Sci Adv, 2020-04-01;6(14):eaay2801.
    Species: Mouse
    Sample Types: Serum
  20. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice
    Authors: C Quach, Y Song, H Guo, S Li, H Maazi, M Fung, N Sands, D O'Connell, S Restrepo-V, B Chai, D Nemecio, V Punj, O Akbari, GE Idos, SM Mumenthale, N Wu, SE Martin, A Hagiya, J Hicks, H Cui, C Liang
    Nat Commun, 2019-12-12;10(1):5681.
    Species: Mouse
    Sample Types: Serum
  21. Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice
    Authors: A Miyauchi, W Watanabe, T Akashi, S Hashiguchi, H Yoshida, C Sugita, M Kurokawa
    Toxicol Rep, 2019-05-31;6(0):514-520.
    Species: Mouse
    Sample Types: Bronchoalveolar lavage fluid
  22. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade
    Authors: JJ Ishizuka, RT Manguso, CK Cheruiyot, K Bi, A Panda, A Iracheta-V, BC Miller, PP Du, KB Yates, J Dubrot, I Buchumensk, DE Comstock, FD Brown, A Ayer, IC Kohnle, HW Pope, MD Zimmer, DR Sen, SK Lane-Retic, EJ Robitschek, GK Griffin, NB Collins, AH Long, JG Doench, D Kozono, EY Levanon, WN Haining
    Nature, 2018-12-17;565(7737):43-48.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse IFN-beta Quantikine ELISA Kit

Average Rating: 4.5 (Based on 6 Reviews)

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Mouse IFN-beta Quantikine ELISA Kit
By Anonymous on 02/07/2023
Sample Tested: Mouse spleenocytes
Mouse IFN-beta Quantikine ELISA Kit
By James AHODANTIN on 11/15/2022
Sample Tested: EDTA Plasma
Mouse IFN-beta Quantikine ELISA Kit MIFNB0
Mouse IFN-beta Quantikine ELISA Kit
By Anonymous on 10/26/2022
Sample Tested: Cell culture supernatant

Cell culture supernatants transfected with human and mouse IFNb on BHK-21 cells

Mouse IFN-beta Quantikine ELISA Kit MIFNB0
Mouse IFN-beta Quantikine ELISA Kit
By Yan Wang on 11/15/2019
Sample Tested: IC-21 mouse macrophage cell line
Mouse IFN-beta Quantikine ELISA Kit
By Adam Guess on 10/19/2017
Sample Tested: Cell Culture Supernates
Mouse IFN-beta Quantikine ELISA Kit MIFNB0
Mouse IFN-beta Quantikine ELISA Kit
By Christine Quach on 09/07/2017
Sample Tested: Cell culture supernatant,Serum