Mouse IFN-gamma Fluorescein-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IFN‑ gamma in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Fluorescein-conjugated Monoclonal Antibody (Catalog # IC485F) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC006F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of IFN‑ gamma in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) stimulated for 4 hours with Cell Activation Cocktail 500x (5476) or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Fluorescein-conjugated Monoclonal Antibody (Catalog # IC485F) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (FAB554A). Quadrant markers were set based on control antibody staining (IC006F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IFN-gamma
Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
Product Datasheets
Citations for Mouse IFN-gamma Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism
Authors: Fernando, V;Zheng, X;Sharma, V;Furuta, S;
bioRxiv : the preprint server for biology
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Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue
Acta Neuropathol Commun, 2016-09-06;4(1):100.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Functional relevance of the IL-23-IL-17 axis in lungs in vivo.
Authors: Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhall C, Sjostrand M, Kolls JK, Anderson GP, Linden A
Am. J. Respir. Cell Mol. Biol., 2006-12-01;36(4):442-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
An HLA-A2.1-transgenic rabbit model to study immunity to papillomavirus infection.
Authors: Hu J, Peng X, Schell TD, Budgeon LR, Cladel NM, Christensen ND
J. Immunol., 2006-12-01;177(11):8037-45.
Species: Rabbit
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator.
Authors: Chiang YM, Chang CL, Chang SL, Yang WC, Shyur LF
J Ethnopharmacol, 2006-10-19;110(3):532-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.
Authors: Utting O, Sedgmen BJ, Watts TH, Shi X, Rottapel R, Iulianella A, Lohnes D, Veillette A
Mol. Cell. Biol., 2004-07-01;24(13):6067-75.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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