Mouse IFN-gamma PE-conjugated Antibody

Catalog #: IC485P
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC485P-100
IC485P-025

Detection of IFN‑ gamma in Mouse Splenocytes Stimulated to Induce Th1 cells by Flow Cytometry.

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All IFN-gamma Antibodies

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Citations (2)

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Mouse IFN-gamma PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN‑ gamma, rrIFN‑ gamma, rpIFN‑ gamma, rrmIFN‑ gamma, rfeIFN‑ gamma, or rcaIFN‑ gamma.

Source

Monoclonal Rat IgG_2A Clone # 37895

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IFN-gamma

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IFN-? antibody in Mouse Splenocytes Stimulated to Induce Th1 cells antibody by Flow Cytometry.

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Detection of IFN‑ gamma in Mouse Splenocytes Stimulated to Induce Th1 cells by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC485P) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

View Larger

Detection of IFN‑ gamma in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) stimulated for 4 hours with Cell Activation Cocktail 500x (5476) or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC485P) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (FAB554A). Quadrant markers were set based on control antibody staining (IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name

Interferon gamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse IFN-gamma PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
Authors: G Wang, L Xie, B Li, W Sang, J Yan, J Li, H Tian, W Li, Z Zhang, Y Tian, Y Dai
Nature Communications, 2021-09-30;12(1):5733.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma
Authors: L Ma, M Han, Z Keyoumu, H Wang, S Keyoumu
Med. Sci. Monit., 2017-04-25;23(0):1980-1991.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry