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Mouse IGFBP-5 Antibody

Catalog #: AF578
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF578
AF578-SP
IGFBP‑5 in Mouse Ovary.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFBP-5 in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human IGFBP-5 is observed, and less than 0.3% cross-reactivity with recombinant mouse (rm) IGF‑I, rmIGF-II, rmIGFBP-1, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-5
Val15-Glu271
Accession # Q3UQV0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGFBP‑5 (Catalog # 578-B5)
Immunohistochemistry
5-15 µg/mL
See below

Mouse IGFBP-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IGFBP‑5 Biotinylated Antibody (Catalog # BAF578)

Standard: Recombinant Mouse IGFBP-5 Protein, CF (Catalog # 578-B5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IGFBP‑5 antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr). View Larger

IGFBP‑5 in Mouse Ovary. IGFBP‑5 was detected in perfusion fixed frozen sections of mouse ovary (follicle with oocyte) using 5 µg/mL Goat Anti-Mouse IGFBP‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF578) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse IGFBP-5 by Western Blot View Larger

Detection of Mouse IGFBP-5 by Western Blot Testicular IGFBP-5 levels, measured by Western blotting. Upper: Western blotting of IGFBP-5 and actin in the testes of males homozygous for the deletion allele (Pappa2KO/KO; KO) or the intact conditional allele (Pappa2fl/fl; fl). Lower: Ratio of the intensity of the IGFBP-5 band to that of the actin band, standardized by dividing an individual sample’s ratio by the mean ratio of all samples on the same blot. Horizontal lines represent the means for each group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26416573), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IGFBP-5 by Western Blot View Larger

Detection of Mouse IGFBP-5 by Western Blot Ovarian IGFBP-5 levels, measured by Western blotting. Upper: Western blotting of IGFBP-5 and actin in the ovaries of females homozygous for the deletion allele (Pappa2KO/KO; KO) or the intact conditional allele (Pappa2fl/fl; fl). Lower: Ratio of the intensity of the IGFBP-5 band to that of the actin band, standardized by dividing an individual sample’s ratio by the mean ratio of all samples on the same blot, such that the average value is 1. Horizontal lines represent the means for each group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26416573), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IGFBP-5 by Western Blot View Larger

Detection of Mouse IGFBP-5 by Western Blot Ovarian IGFBP-5 levels, measured by Western blotting. Upper: Western blotting of IGFBP-5 and actin in the ovaries of females homozygous for the deletion allele (Pappa2KO/KO; KO) or the intact conditional allele (Pappa2fl/fl; fl). Lower: Ratio of the intensity of the IGFBP-5 band to that of the actin band, standardized by dividing an individual sample’s ratio by the mean ratio of all samples on the same blot, such that the average value is 1. Horizontal lines represent the means for each group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26416573), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-5

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-5 cDNA encodes a 271 amino acid (aa) residue precursor protein with a putative 19 aa residue signal peptide that is processed to generate the 252 aa residue mature protein. Mouse, human and rat IGFBP-5 share 97% identity. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.

References
  1. James, P.L. et al. (1993) J. Biol. Chem. 268:22305.
  2. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name
Insulin-like Growth Factor Binding Protein 5
Entrez Gene IDs
3488 (Human); 16011 (Mouse)
Alternate Names
IBP5IBP-5; IGF-binding protein 5; IGFBP5; IGFBP-5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5

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Citations for Mouse IGFBP-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. MiR-193b-365, a brown fat enriched microRNA cluster, is essential for brown fat differentiation
    Authors: Lei Sun, Huangming Xie, Marcelo A. Mori, Ryan Alexander, Bingbing Yuan, Shilpa M. Hattangadi et al.
    Nature Cell Biology
  2. Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism
    Authors: R Garcia-Mar, BB Brandao, T Thomou, E Altindis, CR Kahn
    Cell Reports, 2022-01-18;38(3):110277.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Molecular determinants of nephron vascular specialization in the kidney
    Authors: DM Barry, EA McMillan, B Kunar, R Lis, T Zhang, T Lu, E Daniel, M Yokoyama, JM Gomez-Sali, A Sureshbabu, O Cleaver, A Di Lorenzo, ME Choi, J Xiang, D Redmond, SY Rabbany, T Muthukumar, S Rafii
    Nat Commun, 2019-12-13;10(1):5705.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The putative role of insulin-like growth factor (IGF)-binding protein 5 independent of IGF in the maintenance of pulpal homeostasis in mice
    Authors: K Saito, H Ohshima
    Regen Ther, 2019-09-04;11(0):217-224.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Pappa2 deletion in mice affects male but not female fertility.
    Authors: Christians J, King A, Rogowska M, Hessels S
    Reprod Biol Endocrinol, 2015-09-29;13(0):109.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Synaptic Targets of Medial Septal Projections in the Hippocampus and Extrahippocampal Cortices of the Mouse
    Authors: Gunes Unal, Abhilasha Joshi, Tim J. Viney, Viktor Kis, Peter Somogyi
    The Journal of Neuroscience
  7. MicroRNA-mediated regulation of extracellular matrix formation modulates somatic cell reprogramming
    Authors: Zhonghan Li, Jason Dang, Kung-Yen Chang, Tariq M. Rana
    RNA

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