Mouse IGFBP-6 Antibody

Catalog #: MAB7761 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7761
MAB7761-SP

All IGFBP-6 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGFBP-6 in ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant human (rh) IGFBP-3 and rhIGFBP-5 is observed and no cross-reactivity with rhIGFBP-1, recombinant mouse (rm) IGFBP-1, rhIGFBP-2, rmIGFBP-2, rmIGFBP-3, rhIGFBP-4, rmIGFBP-5, or rhIGFBP-6 is observed.

Source

Monoclonal Rat IgG_2A Clone # 122312

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-6
Gly24-Gly238
Accession # P47880

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IGFBP‑6 (Catalog # 776-B6) under non-reducing conditions only

Mouse IGFBP-6 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IGFBP‑6 Biotinylated Antibody (Catalog # BAF776)

Standard: Recombinant Mouse IGFBP-6 Protein, CF (Catalog # 776-B6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) precursor protein with a putative 25 aa signal peptide that is processed to generate the 213 aa mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% aa similarity. Mouse and rat IGFBP-6 share 94% aa similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo.

References

Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Long Name

Insulin-like Growth Factor Binding Protein 6

Entrez Gene IDs

3489 (Human); 16012 (Mouse)

Alternate Names

IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6