Home / IgG1 / Mouse IgG1 Antibody

Mouse IgG1 Antibody

Catalog #: MAB9627
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9627-100
Detection of Mouse IgG1Primary Antibodies by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG2B, mouse IgG2A, and mouse IgG3 was observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2213C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IgG<sub>1</sub>Primary Antibodies antibody by Western Blot. View Larger

Detection of Mouse IgG1Primary Antibodies by Western Blot. Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG1isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG1(Catalog # MAB9627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG1isotype antibody at approximately 39 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG1Monoclonal Antibody (Catalog # MAB9627). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Mouse IgG<sub>1</sub>Primary Antibody by Anti-Mouse IgG<sub>1</sub>-specific Secondary Antibody. View Larger

Detection of Mouse IgG1Primary Antibody by Anti-Mouse IgG1-specific Secondary Antibody. Human PBMC lymphocytes were stained with Mouse anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, Mouse IgG1isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2Bisotype), followed by Rabbit anti-Mouse IgG1-specific secondary antibody (Catalog # MAB9627) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Mouse IgG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Blocking insulin-like growth factor 1 receptor signaling pathway inhibits neuromuscular junction regeneration after botulinum toxin-A treatment
    Authors: Ishihara, H;Otani, Y;Tanaka, K;Miyajima, H;Ngo, HX;Fujitani, M;
    Cell death & disease
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo Control

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse IgG1 Antibody

There are currently no reviews for this product. Be the first to review Mouse IgG1 Antibody and earn rewards!

Have you used Mouse IgG1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review