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Mouse IgG3 Isotype Control

Catalog #: MAB007
10 Citations Datasheet / COA / SDS
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MAB007
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG3 Isotype Control Summary

Specificity
Selected for use as a mouse IgG3 isotype control. Recognizes KLH.
Source
Monoclonal Mouse IgG3 Clone # 133316
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213; FLJ39988; FLJ40036; FLJ40253; FLJ40587; FLJ40789; FLJ40834; IgG3; immunoglobulin heavy constant gamma 3 (G3m marker); MGC45809

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Citations for Mouse IgG3 Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. MANF protein expression is upregulated in immune cells in the ischemic human brain and systemic recombinant MANF delivery in rat ischemic stroke model demonstrates anti-inflammatory effects
    Authors: Anttila, JE;Mattila, OS;Liew, HK;Mätlik, K;Mervaala, E;Lindholm, P;Lindahl, M;Lindsberg, PJ;Tseng, KY;Airavaara, M;
    Acta neuropathologica communications
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. MUC20 expression marks the receptive phase of the human endometrium
    Authors: A Stepanjuk, M Koel, M Pook, M Saare, K Jääger, M Peters, K Krjutškov, S Ingerpuu, A Salumets
    Reprod. Biomed. Online, 2019-05-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats
    Authors: C Celik-Ozen
    Andrology, 2016-12-19;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. ChemR23, the receptor for chemerin and resolvin E1, is expressed and functional on M1 but not on M2 macrophages.
    Authors: Herova M, Schmid M, Gemperle C, Hersberger M
    J Immunol, 2015-01-30;194(5):2330-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  5. Poly(ADP-ribose) polymerase inhibition improves endothelin-1-induced endothelial dysfunction in rat thoracic aorta
    Authors: Bedriniam Yilmaz, Pinar Sahin, Ece Ordueri, Ciler Celik-Ozenci, Arda Tasatargil
    Upsala Journal of Medical Sciences
  6. Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction
    Authors: Orcun Celik, Omer Kutlu, Merih Tekcan, Ciler Celik-Ozenci, Ismail T T Koksal
    Asian Journal of Andrology
  7. Resolvin E1 and chemokine-like receptor 1 mediate bone preservation.
    Authors: Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R
    J Immunol, 2012-12-14;190(2):689-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2012-11-27;21(1):228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  9. Arginine Methyltransferases Are Regulated by Epstein-Barr Virus in B Cells and Are Differentially Expressed in Hodgkin’s Lymphoma
    Authors: Sarah Leonard, Naheema Gordon, Nikki Smith, Martin Rowe, Paul G. Murray, Ciarán B. Woodman
    Pathogens
  10. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006-08-10;355(1):6-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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