Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Catalog # Availability Size / Price Qty
IC003R
R&D Systems Antibodies
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Product Details
Citations (6)
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Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. MYC disrupts transcriptional and metabolic circadian oscillations in cancer and promotes enhanced biosynthesis
    Authors: Juliana Cazarin, Rachel E. DeRollo, Siti Noor Ain Binti Ahmad Shahidan, Jamison B. Burchett, Daniel Mwangi, Saikumari Krishnaiah et al.
    PLOS Genetics
  2. MYC disrupts transcriptional and metabolic circadian oscillations in cancer and promotes enhanced biosynthesis
    Authors: Rachel E Cazarin, Rachel E. DeRollo, Siti Noor Ain Binti Ahmad Shahidan, Jamison B. Burchett, Daniel Mwangi, Saikumari Krishnaiah et al.
    bioRxiv
  3. The inhibitory receptors PD1, Tim3, and A2aR are highly expressed during mesoCAR T cell manufacturing in advanced human epithelial ovarian cancer
    Authors: Akbari, B;Soltantoyeh, T;Shahosseini, Z;Yarandi, F;Hadjati, J;Mirzaei, HR;
    Cancer cell international
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  4. Impact of delta9-Tetrahydrocannabinol on Rheumatoid Arthritis Synovial Fibroblasts Alone and in Co-Culture with Peripheral Blood Mononuclear Cells
    Authors: T Lowin, C Kok, S Smutny, G Pongratz
    Biomedicines, 2022-05-11;10(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  5. Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2
    Authors: L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu
    Nature Communications, 2022-01-20;13(1):405.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Leukocyte TNFR1 and TNFR2 Expression Contributes to the Peripheral Immune Response in Cases with Ischemic Stroke
    Authors: RB Hansen, CCH Laursen, N Nawaz, JS Madsen, HH Nielsen, C Kruuse, A Møller, M Degn, KL Lambertsen
    Cells, 2021-04-09;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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