Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 20102
View all IgG2A Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as mouse IgG2A isotype control for flow cytometry applications.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Mouse IgG2A Fluorescein-conjugated Antibody

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB330F, filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC0003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IgG2A Fluorescein-conjugated Antibody

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 2 reviews rated 4.5 using IC003F in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Mouse IgG2A Fluorescein-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2A Fluorescein-conjugated Antibody

For research use only

Citations for Mouse IgG2A Fluorescein-conjugated Antibody

Customer Reviews for Mouse IgG2A Fluorescein-conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Mouse IgG2A Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 02/02/2023
    Use as Iso-type Ctrl
    Mouse IgG2A Fluorescein-conjugated Antibody IC003F
  • Mouse IgG2A Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 07/07/2017

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FAQs

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View all FAQs for Isotype Controls