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Mouse IgG2A Fluorescein-conjugated Antibody

Catalog #: IC003F
15 Citations 2 Reviews Datasheet / COA / SDS
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IC003F
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2A Fluorescein-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB330F, filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC0003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Leptin-producing monocytes in the airway submucosa may contribute to asthma pathogenesis
    Authors: K Watanabe, M Suzukawa, S Kawauchi-W, S Igarashi, I Asari, S Imoto, H Tashimo, T Fukami, A Hebisawa, S Tohma, T Nagase, K Ohta
    Respiratory investigation, 2022-11-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
  2. Age‐related macular degeneration and myeloproliferative neoplasms – A common pathway
    Authors: Charlotte Liisborg
    Acta Ophthalmologica
  3. Interleukin-6 Signaling in Triple Negative Breast Cancer Cells Elicits the Annexin A1/Formyl Peptide Receptor 1 Axis and Affects the Tumor Microenvironment
    Authors: L Vecchi, STS Mota, MAP Zóia, IC Martins, JB de Souza, TG Santos, AO Beserra, VP de Andrade, LR Goulart, TG Araújo
    Cells, 2022-05-20;11(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  4. Annexin A1 promotes the nuclear localization of the epidermal growth factor receptor in castration-resistant prostate cancer
    Authors: STS Mota, L Vecchi, DA Alves, AO Cordeiro, GS Guimarães, E Campos-Fer, YCP Maia, BC Dornelas, SM Bezerra, VP de Andrade, LR Goulart, TG Araújo
    Int. J. Biochem. Cell Biol., 2020-08-25;127(0):105838.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  5. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019-10-22;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Bortezomib improves adoptive carbonic anhydrase�IX‑specific chimeric antigen receptor‑modified NK92 cell therapy in mouse models of human renal cell carcinoma
    Authors: Qing Zhang, Jinjing Xu, Jiage Ding, Hongyan Liu, Huizhong Li, Hailong Li et al.
    Oncology Reports
  7. Expression of ABCG 2 and B mi‐1 in oral potentially malignant lesions and oral squamous cell carcinoma
    Authors: Andrew J. Dalley, Luke P. Pitty, Aidan G. Major, Ahmad A. AbdulMajeed, Camile S. Farah
    Cancer Medicine
  8. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011-07-25;6(7):e22504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  9. Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal
    Authors: Ying I. Wang, John Schulze, Nadine Raymond, Tyler Tomita, Kayan Tam, Scott I. Simon et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  10. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009-08-01;127(4):549-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  11. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008-11-01;38(11):3050-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  12. Sinomenine suppresses TNF-alpha-induced VCAM-1 expression in human umbilical vein endothelial cells.
    Authors: Huang J, Lin Z, Luo M, Lu C, Kim MH, Yu B, Gu J
    J Ethnopharmacol, 2007-08-11;114(2):180-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. HIF-1 and NF-kappaB-mediated upregulation of CXCR1 and CXCR2 expression promotes cell survival in hypoxic prostate cancer cells.
    Authors: Maxwell PJ, Gallagher R, Seaton A, Wilson C, Scullin P, Pettigrew J, Stratford IJ, Williams KJ, Johnston PG, Waugh DJ
    Oncogene, 2007-05-28;26(52):7333-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  14. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
    Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
    Eur. J. Immunol., 2005-09-01;35(9):2573-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  15. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress.
    Authors: Wang YI, Bettaieb A, Sun C et al.
    PLoS One.

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Reviews for Mouse IgG2A Fluorescein-conjugated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Mouse IgG2A Fluorescein-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

Use as Iso-type Ctrl

Flow Cytometry Mouse IgG2A Fluorescein-conjugated Antibody IC003F
Mouse IgG2A Fluorescein-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human