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Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Catalog #: IC0041G
14 Citations 2 Reviews Datasheet / COA / SDS
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IC0041G
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (14)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control Summary

Specificity
Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood mononuclear cell (PBMCs) were stained with Mouse Anti-Human Plexin A1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB65361G, filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells
    Authors: Morimoto Y, Yamashita N, Daimon T et al.
    Journal for immunotherapy of cancer
  2. Coagulation potential and the integrated omics of extracellular vesicles from COVID-19 positive patient plasma
    Authors: S Setua, K Thangaraju, M Dzieciatko, RB Wilkerson, T Nemkov, DR Lamb, Y Tagaya, T Boyer, T Rowden, A Doctor, A D'Alessand, PW Buehler
    Scientific Reports, 2022-12-23;12(1):22191.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Macrophage-derived exosomal miR-342-3p promotes the progression of renal cell carcinoma through the NEDD4L/CEP55 axis
    Authors: JIAFU FENG, BEI XU, CHUNMEI DAI, YAODONG WANG, GANG XIE, WENYU YANG et al.
    Oncology Research
  4. M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties
    Authors: Guifang Zhao, Lijuan Ding, Hongquan Yu, Weiyao Wang, Huan Wang, Yao Hu et al.
    Cell Death Discovery
  5. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
    Authors: BRB Pires, R Binato, GM Ferreira, S Corrêa, B Du Rocher, D Bulzico, S Crocamo, EC Dos Santos, LG Lima, E Abdelhay
    International Journal of Molecular Sciences, 2021-11-10;22(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  6. Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor &beta and S100A8/9 up-regulation
    Authors: Z Wang, G Zheng, G Li, M Wang, Z Ma, H Li, XY Wang, H Yi
    J Cell Mol Med, 2020-10-23;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer
    Authors: Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri
    Cancer Discov, 2020-05-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation
    Authors: YL Kuang, A Munoz, G Nalula, KE Santostefa, V Sanghez, G Sanchez, N Terada, AN Mattis, M Iacovino, C Iribarren, RM Krauss, MW Medina
    Stem Cell Res, 2019-04-10;37(0):101434.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. A PKA/cdc42 Signaling Axis Restricts Angiogenic Sprouting by Regulating Podosome Rosette Biogenesis and Matrix Remodeling
    Authors: JL MacKeil, P Brzezinska, J Burke-Klei, AW Craig, CJB Nicol, DH Maurice
    Sci Rep, 2019-02-20;9(1):2385.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Transcript- and protein-level analyses of the response of human eosinophils to glucocorticoids
    Authors: Manasi Gadkari, Michelle A. Makiya, Fanny Legrand, Kindra Stokes, Thomas Brown, Katherine Howe et al.
    Scientific Data
  11. DSPP-MMP20 gene silencing downregulates cancer stem cell markers in human oral cancer cells
    Authors: Nikolaos G. Nikitakis, Ioannis Gkouveris, Jaya Aseervatham, Kelvin Barahona, Kalu U. E. Ogbureke
    Cellular & Molecular Biology Letters
  12. Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells
    Authors: Y Shlyakhtin, V Pavet, H Gronemeyer
    Cell Death Dis, 2017-08-31;8(8):e3025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Tumour-associated macrophages secrete pleiotrophin to promote PTPRZ1 signalling in glioblastoma stem cells for tumour growth
    Authors: Y Shi, YF Ping, W Zhou, ZC He, C Chen, BS Bian, L Zhang, L Chen, X Lan, XC Zhang, K Zhou, Q Liu, H Long, TW Fu, XN Zhang, MF Cao, Z Huang, X Fang, X Wang, H Feng, XH Yao, SC Yu, YH Cui, X Zhang, JN Rich, S Bao, XW Bian
    Nat Commun, 2017-06-01;8(0):15080.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Base-modified UDP-sugars reduce cell surface levels of P-selectin glycoprotein 1 (PSGL-1) on IL-1?-stimulated human monocytes
    Authors: Varsha Kanabar
    Glycobiology, 2016-05-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control
By Anonymous on 08/25/2021
Application: Flow Sample Tested: IPS2 induced pluripotent stem cells Species: Human
Flow Cytometry Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control IC0041G
Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control
By Mike van Nijmegen on 08/10/2021
Application: Flow Sample Tested: HK-2 human proximal tubule epithelial cell line Species: Human and Mouse
Flow Cytometry Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control IC0041G