Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Catalog # Availability Size / Price Qty
IC0041N
R&D Systems Antibodies
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control Summary

Specificity
Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

Product Datasheets

You must select a language.

x

Citation for Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2
    Authors: Qiao Lu, Jia Liu, Shuai Zhao, Maria Florencia Gomez Castro, Maudry Laurent-Rolle, Jianbo Dong et al.
    Immunity

FAQs

No product specific FAQs exist for this product, however you may

View all Isotype Control FAQs
Loading...

Reviews for Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control
By Harry Hurley on 12/04/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

5 uL of IC0041N added to PBMC stained with BDCA2-PerCP/Cy5.5, CD123-BV510, CD3-PE, CD14-APC/Cy7. Data shown is gated pDC (BDCA2+CD123+).