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Mouse IgG3 Alexa Fluor® 647-conjugated Antibody

Catalog #: IC007R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC007R
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Mouse IgG3 Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG3 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG3 Clone # 133316
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1435R, filled histogram) or Mouse IgG Alexa Fluor® 647-conjugated Isotype Control Antibody (Catalog # IC007R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213; FLJ39988; FLJ40036; FLJ40253; FLJ40587; FLJ40789; FLJ40834; IgG3; immunoglobulin heavy constant gamma 3 (G3m marker); MGC45809

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FAQs

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