Mouse IGSF2/CD101 APC-conjugated Antibody

Catalog #: FAB3368A Datasheet / COA / SDS

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FAB3368A

Detection of IGSF2/CD101 in Mouse GR-1 Positive Blood Cells by Flow Cytometry.

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All IGSF2/CD101 Antibodies

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Mouse IGSF2/CD101 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGSF2/CD101 in flow cytometry.

Source

Monoclonal Rat IgG_2A Clone # 307707

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse IGSF2/CD101
Gln21-Tyr1033
Accession # A8E0Y8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IGSF2/CD101 in Mouse GR-1 Positive Blood Cells by Flow Cytometry. Mouse GR-1 positive blood cells were stained with Rat Anti-Mouse IGSF2/CD101 APC-conjugated Monoclonal Antibody (Catalog # FAB3368A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IGSF2/CD101

IGSF2, also known as CD101 and V7 is a 135-140 kDa member of the EWI family, Ig superfamily of molecules. It is a type I transmembrane glycoprotein that is expressed on dermal dendritic cells (DCs), granulocytes, monocytes, activated T cells, and Tregs. IGSF2 ligation is involved in T cell activation. On dermal DCs, CD101 ligation induces IL-10 expression, and on activated T cells, CD101 ligation blocks IL-2 expression, two effects that downregulate T cell activity. Notably, in mouse, CD101 expression on CD62L⁺⁺ Tregs identifies a population of cells that have potent suppressor activity. The extracellular domain of mouse shares 86% and 69% amino acid sequence identity with the ECD of rat and human CD101, respectively.

Long Name

Immunoglobulin Superfamily, Member 2

Entrez Gene IDs

9398 (Human); 630146 (Mouse)

Alternate Names

CD101 antigen; CD101 molecule; CD101; Cell surface glycoprotein V7; EWI101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; IGSF2; Leukocyte Surface Protein; member 2; V7