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Mouse IGSF2/CD101 Biotinylated Antibody

Catalog #: BAM3368
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BAM3368
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Citations (1)
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Mouse IGSF2/CD101 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGSF2/CD101.
Source
Monoclonal Rat IgG2A Clone # 307707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse IGSF2/CD101
Arg21-Leu1033
Accession # NP_997088
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse blood GR‑1+ granulocytes
Immunocytochemistry
8-25 µg/mL
Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF2/CD101

IGSF2, also known as CD101, is an Ig-superfamily transmembrane protein. It is expressed on dendritic cells, Langerhans cells, granulocytes, and activated T cells. IGSF2 ligation is involved in T cell activation. Polymorphisms in IGSF2 are associated with susceptibility to type I diabetes. The extracellular domains of mouse and human IGSF2 share 70% amino acid sequence identity.

Long Name
Immunoglobulin Superfamily, Member 2
Entrez Gene IDs
9398 (Human); 630146 (Mouse)
Alternate Names
CD101 antigen; CD101 molecule; CD101; Cell surface glycoprotein V7; EWI101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; IGSF2; Leukocyte Surface Protein; member 2; V7

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Citation for Mouse IGSF2/CD101 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules.
    Authors: Calderon B, Suri A, Miller MJ
    Proc. Natl. Acad. Sci. U.S.A., 2008-04-21;105(16):6121-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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