Mouse IGSF9 Antibody

Catalog #: MAB9140 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9140-100
MAB9140-SP

Detection of IGSF9 in HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGSF9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGSF9 in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 993107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGSF9
Arg21-Leu730
Accession # Q05BQ1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IGSF9 in HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP by Flow Cytometry. HEK293 human cell line transfected with (A) Mouse IGSF9 or (B) irrelevant protein, and eGFP was stained with Rat Anti-Mouse IGSF9 Monoclonal Antibody (Catalog # MAB9140) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on Rat IgG2B Isotype Control Antibody (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF9

IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).

References

Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
Doudney, K. et al. (2002) Genomics 79:663.
Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
Mishra, A. et al. (2014) J. Neurosci. 34:4187.
Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.

Long Name

Immunoglobulin Superfamily, Member 9

Entrez Gene IDs

57549 (Human); 93842 (Mouse); 304982 (Rat)

Alternate Names

Dasm1; FP18798; IGSF9; IGSF9A; immunoglobulin superfamily member 9A; immunoglobulin superfamily, member 9; KIAA1355; Nrt1; Protein Turtle Homolog A; TUTLA