Mouse IKK beta Antibody

Catalog #: MAB7155
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7155
MAB7155-SP

Detection of Mouse IKK beta by Western Blot.

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Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IKK beta Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IKK beta in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 725818

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IKK beta
Val530-Asp757
Accession # O88351

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse IKK beta by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IKK beta Monoclonal Antibody (Catalog # MAB7155) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK beta

I kappa B kinase beta (IKK beta ) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF kappa B. Like IKK alpha, IKK beta contains kinase (aa 15-300), leucine zipper (aa 458‑479), and helix-loop-helix (aa 605-644) domains. NF kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha /IKK beta heterodimers. Within amino acids 530‑757, mouse IKKb shares 89% and 96% aa sequence identity with human and rat IKKb, respectively.

Long Name

IkB Kinase beta

Entrez Gene IDs

3551 (Human); 16150 (Mouse); 84351 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK2; IKK2FLJ38368; IKK-B; IKK-betaFLJ36218; IKKBFLJ40509; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; NFKBIKBFLJ33771; Nuclear factor NF-kappa-B inhibitor kinase beta

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IKK beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Mitochondrial permeabilization engages NF-kappa B-dependent anti-tumour activity under caspase deficiency
Authors: Evangelos Giampazolias, Barbara Zunino, Sandeep Dhayade, Florian Bock, Catherine Cloix, Kai Cao et al.
Nature Cell Biology
IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis
Authors: H Fujita, T Fujita, H Fujii
Cells, 2022-04-25;11(9):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Uncaria tomentosa improves insulin sensitivity and inflammation in experimental NAFLD
Authors: LCC Araujo, KB Feitosa, GM Murata, IC Furigo, SA Teixeira, CF Lucena, LM Ribeiro, MN Muscará, SKP Costa, J Donato, S Bordin, R Curi, CRO Carvalho
Sci Rep, 2018-07-20;8(1):11013.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
IL6 mediated inflammatory loop reprograms normal to EMT(+) metastatic CSCs in pre-neoplastic liver of TGF? deficient ?2SP(+/-) mice
Authors: Abhisek Mitra
Hepatology, 2017-01-20;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V
Nature, 2015-12-09;528(7582):370-5.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Epigenetic Roles of MLL Oncoproteins Are Dependent on NF-kappa B
Authors: Hsu-Ping Kuo, Zhong Wang, Dung-Fang Lee, Masayuki Iwasaki, Jesus Duque-Afonso, Stephen H.K. Wong et al.
Cancer Cell