Mouse IL-10 R beta Antibody

Catalog #: MAB53681
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB53681
MAB53681-SP

Detection of IL‑10 R beta in EL‑4 Mouse Cell Line by Flow Cytometry.

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All IL-10 R beta Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-10 R beta Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-10 R beta in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 547324

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta
Ile21-Ser220
Accession # Q61190

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑10 R beta in EL‑4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL-10 R beta Monoclonal Antibody (Catalog # MAB53681, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R beta

IL-10 R beta (Interleukin 10 receptor beta; also IL-10R2, CD210b and CRF2-4) is an 80-85 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B, and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein with an extracellular domain that possesses two fibronectin type III domains (aa 22-107 and
111‑208). There is one alternate start site at Met20. Over aa 21- 220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta, respectively.

Long Name

Interleukin 10 Receptor beta

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Alternate Names

CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4

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Citation for Mouse IL-10 R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1