Mouse IL-12 p70 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 36 | 44 | 36 |
Mean (pg/mL) | 28 | 68 | 193 | 20 | 56 | 230 |
Standard Deviation | 2.5 | 6.5 | 19.4 | 2.3 | 6.2 | 20 |
CV% | 8.9 | 9.6 | 10.1 | 11.5 | 11.1 | 8.7 |
Recovery
The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=7) | 101 | 95-106 |
Serum (n=7) | 97 | 88-104 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-12 p70
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse IL-12 p70 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The Protective Role of Troxerutin (Trox) in Counteracting Anaplastic Thyroid Carcinoma (ATC) Progression
Authors: Bova, V;Basilotta, R;Casili, G;Lanza, M;Filippone, A;Campolo, M;Capra, AP;Vitale, G;Chisari, G;Colarossi, C;Giuffrida, D;Paterniti, I;Esposito, E;
Biomedicines
Species: Human
Sample Types: Cell Culture Supernates
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Leishmania exploits host cAMP/EPAC/calcineurin signaling to induce an IL-33-mediated anti-inflammatory environment for the establishment of infection
Authors: Roy, S;Roy, S;Halder, S;Jana, K;Ukil, A;
The Journal of biological chemistry
Species: Mouse
Sample Types: Cell Culture Supernates
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Intravenous administration of IL-12 encoding self-replicating RNA-lipid nanoparticle complex leads to safe and effective antitumor responses
Authors: Wang, Z;Chen, Y;Wu, H;Wang, M;Mao, L;Guo, X;Zhu, J;Ye, Z;Luo, X;Yang, X;Liu, X;Yang, J;Sheng, Z;Lee, J;Guo, Z;Liu, Y;
Scientific reports
Species: Mouse
Sample Types: Tissue Homogenates
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Decrease in Heparan Sulphate Binding in Tropism-Retargeted Oncolytic Herpes Simplex Virus (ReHV) Delays Blood Clearance and Improves Systemic Anticancer Efficacy
Authors: Vannini, A;Parenti, F;Forghieri, C;Vannini, G;Barboni, C;Zaghini, A;Gianni, T;Campadelli-Fiume, G;
Cancers
Species: Mouse
Sample Types: Cell Culture Supernates
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An NFAT1-C3a-C3aR Positive Feedback Loop in Tumor-Associated Macrophages Promotes a Glioma Stem Cell Malignant Phenotype
Authors: Zhang, Y;Song, Y;Wang, X;Shi, M;Lin, Y;Tao, D;Han, S;
Cancer immunology research
Species: Mouse
Sample Types: Cell Culture Supernates
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METTL3-mediated m6A methylation regulates ovarian cancer progression by recruiting myeloid-derived suppressor cells
Authors: Wang, J;Ling, D;Shi, L;Li, H;Peng, M;Wen, H;Liu, T;Liang, R;Lin, Y;Wei, L;Zhang, G;Chen, S;
Cell & bioscience
Species: Mouse, Transgenic Mouse
Sample Types: Serum, Peritoneal Lavage Fluid
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Immune-Stimulating Potential of Lacticaseibacillus rhamnosus LM1019 in RAW 264.7 Cells and Immunosuppressed Mice Induced by Cyclophosphamide
Authors: You, Y;Kim, SH;Kim, CH;Kim, IH;Shin, Y;Kim, TR;Sohn, M;Park, J;
Microorganisms
Species: Mouse
Sample Types: Serum
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Fallopia japonica Root Extract Ameliorates Ovalbumin-Induced Airway Inflammation in a CARAS Mouse Model by Modulating the IL-33/TSLP/NF-?B Signaling Pathway
Authors: Jin, J;Fan, YJ;Nguyen, TV;Yu, ZN;Song, CH;Lee, SY;Shin, HS;Chai, OH;
International journal of molecular sciences
Species: Mouse
Sample Types: BALF, NLF
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The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation
Authors: KJ Smith, D Minns, BJ McHugh, RK Holloway, R O'Connor, A Williams, L Melrose, R McPherson, VE Miron, DJ Davidson, E Gwyer Find
PloS Biology, 2022-08-26;20(8):e3001554.
Species: Mouse
Sample Types: CSF
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Activation of TLR-pathway to induce host Th1 immune response against visceral leishmaniasis: Involvement of galactosylated-flavonoids
Authors: S Pradhan, Snehlata, D Manna, S Karmakar, MK Singh, A Bhattachar, B Mukherjee, J Paul
Heliyon, 2022-07-03;8(7):e09868.
Species: Mouse
Sample Types: Cell Culture Supernates
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Liposomal beta-Sitosterol Suppresses Metastasis of CT26/luc Colon Carcinoma via Inhibition of MMP-9 and Evoke of Immune System
Authors: CY Shen, CF Lee, WT Chou, JJ Hwang, YS Tyan, HY Chuang
Pharmaceutics, 2022-06-07;14(6):.
Species: Mouse
Sample Types: Tissue Homogenates
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Functional insights into Brucella transcriptional regulator ArsR
Authors: C Shi, S Wang, J Han, L Xi, M Li, Z Li, H Zhang
Microbial pathogenesis, 2022-05-24;168(0):105557.
Species: Mouse
Sample Types: Cell Culture Supernates
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Nociceptor-derived Reg3gamma prevents endotoxic death by targeting kynurenine pathway in microglia
Authors: E Sugisawa, T Kondo, Y Kumagai, H Kato, Y Takayama, K Isohashi, E Shimosegaw, N Takemura, Y Hayashi, T Sasaki, MM Martino, M Tominaga, K Maruyama
Cell Reports, 2022-03-08;38(10):110462.
Species: Mouse
Sample Types: Plasma
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Engineering immunoproteasome-expressing mesenchymal stromal cells: A potent cellular vaccine for lymphoma and melanoma in mice
Authors: Jamilah Abusarah, Fatemeh Khodayarian, Nehme El-Hachem, Natasha Salame, Martin Olivier, Mohammad Balood, Katiane Roversi, Sebastien Talbot, Jean-Pierre Bikorimana, Jingkui Chen, Mario Jolicoeur, Louis-Eric Trudeau, Samaneh Kamyabiazar, Borhane Annabi, Francis Robert, Jerry Pelletier, Abed-El-Hakim El-Kadiry, Riam Shammaa, Moutih Rafei
Cell Rep Med
Species: Mouse
Sample Types: Cell Culture Supernates
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Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study
Authors: S Kos, M Bosnjak, T Jesenko, B Markelc, U Kamensek, K Znidar, U Matkovic, A Rencelj, G Sersa, R Hudej, A Tuljak, M Peterka, M Cemazar
Pharmaceutics, 2021-10-19;13(10):.
Species: Mouse
Sample Types: Cell Cultue Supernates
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Mechanism of chlorogenic acid in alveolar macrophage polarization in Klebsiella pneumoniae-induced pneumonia
Authors: QR Li, SR Tan, L Yang, W He, L Chen, FX Shen, Z Wang, HF Wang
Journal of leukocyte biology, 2021-09-29;0(0):.
Species: Mouse
Sample Types: BALF
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Pore-forming alpha-hemolysin efficiently improves the immunogenicity and protective efficacy of protein antigens
Authors: JT Zou, HM Jing, Y Yuan, LH Lei, ZF Chen, Q Gou, QS Xiong, XL Zhang, Z Zhao, XK Zhang, H Zeng, QM Zou, JY Zhang
PloS Pathogens, 2021-07-21;17(7):e1009752.
Species: Mouse
Sample Types: Tissue Homogenates
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Nanoparticle-mediated tumor cell expression of mIL-12 via systemic gene delivery treats syngeneic models of murine lung cancers
Authors: HH Ahn, C Carrington, Y Hu, HW Liu, C Ng, H Nam, A Park, C Stace, W West, HQ Mao, MG Pomper, CG Ullman, I Minn
Scientific Reports, 2021-05-06;11(1):9733.
Species: Mouse
Sample Types: Cell Culture Supernates
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Phagocytosis and macrophage polarization on bacterially contaminated dental implant materials and effects on tissue integration
Authors: Y Yuan, Y Ren, M Dijk, GI Geertsema-, J Atema-Smit, HJ Busscher, HC van der Me
European cells & materials, 2021-03-30;41(0):421-430.
Species: Mouse
Sample Types: Cell Culture Supernates
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Oral administration of eugenol oleate cures experimental visceral leishmaniasis through cytokines abundance
Authors: MR Charan Raj, A Kar, S Srinivasan, D Chellappan, J Debnath, S Kar Mahapa
Cytokine, 2020-10-28;0(0):155301.
Species: Mouse
Sample Types: Cell Culture Supernates
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Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
Authors: K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai
Cell Rep, 2020-07-14;32(2):107906.
Species: Mouse
Sample Types: Serum
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Combined administration of SHP2 inhibitor SHP099 and the &alpha7nAChR agonist PNU282987 protect mice against DSS?induced colitis
Authors: J Xiao, G Zhang, S Gao, J Shen, H Feng, Z He, C Xu
Mol Med Rep, 2020-07-10;22(3):2235-2244.
Species: Mouse
Sample Types: Cell Culture Supernates
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Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state
Authors: T Kawabe, J Yi, A Kawajiri, K Hilligan, D Fang, N Ishii, H Yamane, J Zhu, D Jankovic, KS Kim, G Trinchieri, A Sher
Nat Commun, 2020-07-06;11(1):3366.
Species: Mouse
Sample Types: Cell Culture Supernates
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Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic Signaling
Authors: VA Balaji Rag, M Anwar, MZ Akhter, A Chavez, Y Mao, V Natarajan, S Lakshmikan, M Chrzanowsk, AZ Dudek, L Claesson-W, JK Kitajewski, KK Wary, AB Malik, D Mehta
Cell Rep, 2019-12-10;29(11):3472-3487.e4.
Species: Mouse
Sample Types: Cell Culture Supernates
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Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
Authors: R Leylek, M Alcántara-, Z Lanzar, A Lüdtke, OA Perez, B Reizis, J Idoyaga
Cell Rep, 2019-12-10;29(11):3736-3750.e8.
Species: Mouse
Sample Types: Cell Culture Supernates
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Downregulation of RalGTPase-activating protein promotes colitis-associated cancer via NLRP3 inflammasome activation
Authors: T Iida, Daisuke Hi, N Minami, M Matsuura, K Wagatsuma, K Kawakami, K Nagaishi, M Nojima, H Ikeuchi, S Hirota, R Shirakawa, H Horiuchi, H Nakase
Cell Mol Gastroenterol Hepatol, 2019-10-15;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Oncolytic activity of HF10 in head and neck squamous cell carcinomas
Authors: S Esaki, F Goshima, H Ozaki, G Takano, Y Hatano, D Kawakita, K Ijichi, T Watanabe, Y Sato, T Murata, H Iwata, Y Shibamoto, S Murakami, Y Nishiyama, H Kimura
Cancer Gene Ther., 2019-09-03;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Emodin regulates neutrophil phenotypes to prevent hypercoagulation and lung carcinogenesis
Authors: Z Li, Y Lin, S Zhang, L Zhou, G Yan, Y Wang, M Zhang, M Wang, H Lin, Q Tong, Y Duan, G Du
J Transl Med, 2019-03-18;17(1):90.
Species: Mouse
Sample Types: Alveolar Fluid
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Dendritic cells modulate c-kit expression on the edge between activation and death
Authors: S Simonetti, ABB Seijas, A Natalini, S Vitale, D Runci, A Soriani, A Di Virgili, E Aricò, L Gabriele, A Santoni, F Di Rosa
Eur. J. Immunol., 2019-02-25;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
Redox Biol, 2019-02-06;22(0):101116.
Species: Mouse
Sample Types: Cell Culture Supernates
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Intratumoral expression using a NFkB-based promoter enhances IL12 antitumor efficacy
Authors: G Garaulet, G Pérez-Chac, H Alarcón, A Alfranca, F Mulero, J Martínez-T, JM Zapata, A Rodríguez
Cancer Gene Ther., 2019-01-09;0(0):.
Species: Mouse
Sample Types: Serum
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Non-replicating Newcastle Disease Virus as an adjuvant for DNA vaccine enhances antitumor efficacy through the induction of TRAIL and granzyme B expression
Authors: A Mohebbi, MS Ebrahimzad, S Baghban Ra, M Saeidi, A Tabarraei, SR Mohebbi, S Shirian, A Gorji, A Ghaemi
Virus Res., 2018-12-29;261(0):72-80.
Species: Mouse
Sample Types: Cell Culture Supernates
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Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells
Authors: K Abdi, K Laky, K Padhan, C Petrovas, J Skinner, J Kabat, DW Dorward, J Brzostowsk, EO Long, G Trinchieri, R Varma
J. Immunol., 2018-10-12;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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?-Synuclein disrupts the anti-inflammatory role of Drd2 via interfering ?-arrestin2-TAB1 interaction in astrocytes
Authors: RH Du, Y Zhou, ML Xia, M Lu, JH Ding, G Hu
J Neuroinflammation, 2018-09-10;15(1):258.
Species: Mouse
Sample Types: Cell Culture Supernates
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Regulated intratumoral expression of IL-12 using a RheoSwitch Therapeutic System� (RTS�) gene switch as gene therapy for the treatment of glioma
Authors: JA Barrett, H Cai, J Miao, PD Khare, P Gonzalez, J Dalsing-He, G Sharma, T Chan, LJN Cooper, F Lebel
Cancer Gene Ther., 2018-05-14;0(0):.
Species: Mouse
Sample Types: Serum
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Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS
Authors: HM Song, X Li, YY Liu, WP Lu, ZH Cui, L Zhou, D Yao, HM Zhang
Int. J. Mol. Med., 2018-03-27;0(0):.
Species: Mouse
Sample Types: Serum
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Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)
Authors: BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho
PLoS ONE, 2017-12-13;12(12):e0188779.
Species: Rat
Sample Types: Serum
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Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
J Microbiol Immunol Infect, 2017-06-29;0(0):.
Species: Mouse
Sample Types: BALF
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STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
J. Immunol., 2017-06-02;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
Authors: U Lampreht T, L Loiacono, M Cemazar, U Kamensek, VM Fazio, G Sersa, E Signori
Mediators Inflamm., 2017-05-16;2017(0):5285890.
Species: Mouse
Sample Types: Serum
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Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
Authors: S Ishikawa, T Ishikawa, C Tezuka, K Asano, M Sunagawa, T Hisamitsu
Evid Based Complement Alternat Med, 2017-02-12;2017(0):6054706.
Species: Mouse
Sample Types: Serum
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Microwave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer
Authors: L Li, W Wang, H Pan, G Ma, X Shi, H Xie, X Liu, Q Ding, W Zhou, S Wang
J Transl Med, 2017-01-31;15(1):23.
Species: Mouse
Sample Types: Plasma
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Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer-mediated cancer immunotherapy
Authors: Maja Cemazar
Plasmid, 2016-11-24;89(0):9-15.
Species: Human, Mouse
Sample Types: Cell Culture Supernates
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Subtilisin-like protease-1 secreted through type IV secretion system contributes to high virulence of Streptococcus suis 2
Authors: Supeng Yin
Sci Rep, 2016-06-08;6(0):27369.
Species: Mouse
Sample Types: Serum
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Epigenetic changes in bone marrow progenitor cells influence the inflammatory phenotype and alter wound healing in type 2 diabetes.
Authors: Gallagher K, Joshi A, Carson W, Schaller M, Allen R, Mukerjee S, Kittan N, Feldman E, Henke P, Hogaboam C, Burant C, Kunkel S
Diabetes, 2014-11-03;64(4):1420-30.
Species: Mouse
Sample Types: Cell Culture Supernates
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Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
J Immunol, 2014-07-21;193(5):2330-9.
Species: Mouse
Sample Types: Cell Culture Supernates
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The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
Cytokine, 2014-07-06;69(2):218-25.
Species: Mouse
Sample Types: Cell Culture Supernates
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Leishmania donovani targets tumor necrosis factor receptor-associated factor (TRAF) 3 for impairing TLR4-mediated host response.
Authors: Gupta P, Giri J, Srivastav S, Chande A, Mukhopadhyaya R, Das P, Ukil A
FASEB J, 2014-01-03;28(4):1756-68.
Species: Mouse
Sample Types: Cell Culture Supernates
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Evaluation of p21 promoter for interleukin 12 radiation induced transcriptional targeting in a mouse tumor model.
Authors: Kamensek, Urska, Sersa, Gregor, Cemazar, Maja
Mol Cancer, 2013-11-12;12(1):136.
Species: Mouse
Sample Types: Tissue Homogenates
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Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration.
Authors: Maina, Virginia, Marrella, Veronica, Mantero, Stefano, Cassani, Barbara, Fontana, Elena, Anselmo, Achille, Del Prete, Annalisa, Sozzani, Silvano, Vezzoni, Paolo, Poliani, Pietro L, Villa, Anna
J Leukoc Biol, 2013-09-19;94(6):1221-30.
Species: Mouse
Sample Types: Cell Culture Supernates
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PGI(2) signaling inhibits antigen uptake and increases migration of immature dendritic cells.
Authors: Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, FitzGerald G, Lawson W, Peebles R
J Leukoc Biol, 2013-04-26;94(1):77-88.
Species: Mouse
Sample Types: Cell Culture Supernates
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Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan.
Authors: Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N
J Immunol, 2013-03-20;190(8):4116-28.
Species: Mouse
Sample Types: Cell Culture Supernates
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Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
Authors: Guo Z, Wen Z, Qin A, Zhou Y, Liao Z, Liu Z, Liang Y, Ren T, Xu L
J Immunol, 2013-03-20;190(8):4337-48.
Species: Mouse
Sample Types: Cell Culture Supernates
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TLR-mediated distinct IFN-gamma/IL-10 pattern induces protective immunity against murine visceral leishmaniasis.
Eur. J. Immunol., 2012-08-01;42(8):2087-99.
Species: Mouse
Sample Types: Cell Culture Supernates
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IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
PLoS Pathog., 2012-07-26;8(7):e1002827.
Species: Mouse
Sample Types: Cell Culture Supernates
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The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
PLoS Pathog., 2012-07-12;8(7):e1002799.
Species: Mouse
Sample Types: Serum
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Protein kinase C delta (PKCdelta)-extracellular signal-regulated kinase 1/2 (ERK1/2) signaling cascade regulates glycogen synthase kinase-3 (GSK-3) inhibition-mediated interleukin-10 (IL-10) expression in lipopolysaccharide (LPS)-induced endotoxemia.
Authors: Noh KT, Son KH, Jung ID, Kang HK, Hwang SA, Lee WS, You JC, Park YM
J. Biol. Chem., 2012-03-05;287(17):14226-33.
Species: Mouse
Sample Types: Serum
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The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
J. Immunol., 2011-06-29;187(3):1333-46.
Species: Mouse
Sample Types: Serum
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Improved host defense against septic peritonitis in mice lacking MyD88 and TRIF is linked to a normal interferon response.
Authors: Reim D, Rossmann-Bloeck T, Jusek G, Prazeres da Costa O, Holzmann B
J. Leukoc. Biol., 2011-05-31;90(3):613-20.
Species: Mouse
Sample Types: Plasma
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Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
Am. J. Pathol., 2011-05-10;179(1):302-14.
Species: Mouse
Sample Types: Tissue Homogenates
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Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
Authors: Diao J, Zhao J, Winter E
J. Immunol., 2011-03-23;186(9):5058-67.
Species: Mouse
Sample Types: Cell Culture Supernates
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The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
J. Immunol., 2011-03-09;186(8):4707-15.
Species: Mouse
Sample Types: Serum
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Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
Authors: Wang H, Cheng F
J. Immunol., 2011-03-02;186(0):3986.
Species: Mouse
Sample Types: Cell Culture Supernates
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Anti-inflammatory effects of FTY720 do not prevent neuronal cell loss in a rat model of optic neuritis.
Authors: Rau CR, Hein K, Sattler MB, Kretzschmar B, Hillgruber C, McRae BL, Diem R, Bahr M
Am. J. Pathol., 2011-02-26;178(4):1770-81.
Species: Rat
Sample Types: Tissue Homogenates
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Zoledronic acid impairs myeloid differentiation to tumour-associated macrophages in mesothelioma.
Authors: Veltman JD, Lambers ME, van Nimwegen M
Br. J. Cancer, 2010-07-27;103(5):629-41.
Species: Mouse
Sample Types: Peritoneal Fluid
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Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
J. Immunol., 2010-07-21;185(4):2044-50.
Species: Mouse
Sample Types: Cell Culture Supernates
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Critical role of the disintegrin metalloprotease ADAM17 for intestinal inflammation and regeneration in mice.
Authors: Chalaris A, Adam N, Sina C, Rosenstiel P, Lehmann-Koch J, Schirmacher P, Hartmann D, Cichy J, Gavrilova O, Schreiber S, Jostock T, Matthews V, Hasler R, Becker C, Neurath MF, Reiss K, Saftig P, Scheller J, Rose-John S
J. Exp. Med., 2010-07-05;207(8):1617-24.
Species: Mouse
Sample Types: Cell Culture Supernates
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Oncostatin M induces dendritic cell maturation and Th1 polarization.
Authors: Jung ID, Noh KT, Lee CM, Chun SH, Jeong SK, Park JW, Park WS, Kim HW, Yun CH, Shin YK, Park YM
Biochem. Biophys. Res. Commun., 2010-03-04;394(2):272-8.
Species: Mouse
Sample Types: Cell Culture Supernates
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Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
Cytokine, 2009-12-29;49(3):256-63.
Species: Mouse
Sample Types: BALF
-
The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
Authors: Szymczak WA, Deepe GS
J. Immunol., 2009-07-08;183(3):1964-74.
Species: Mouse
Sample Types: Tissue Homogenates
-
TLR9 activation coupled to IL-10 deficiency induces adverse pregnancy outcomes.
Authors: Thaxton JE, Romero R, Sharma S
J. Immunol., 2009-06-26;183(2):1144-54.
Species: Mouse
Sample Types: Serum
-
A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
J. Immunol., 2009-06-19;183(2):1291-300.
Species: Mouse
Sample Types: BALF
-
TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
Vaccine, 2009-04-03;27(23):3045-52.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis.
Authors: Li H, Ciric B, Yang J, Xu H, Fitzgerald DC, Elbehi M, Fonseca-Kelly Z, Yu S, Zhang GX, Rostami A
J. Neuroimmunol., 2009-02-01;208(1):54-60.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Inhibition of monocyte-derived inflammatory cytokines by IL-25 occurs via p38 Map kinase-dependent induction of Socs-3.
Authors: Caruso R, Stolfi C, Sarra M, Rizzo A, Fantini MC, Pallone F, MacDonald TT, Monteleone G
Blood, 2009-01-07;113(15):3512-9.
Species: Mouse
Sample Types: Serum
-
Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation.
Authors: Li Q, Guo Z, Xu X, Xia S, Cao X
Eur. J. Immunol., 2008-10-01;38(10):2751-61.
Species: Mouse
Sample Types: BALF
-
Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
J. Immunol., 2008-09-15;181(6):4208-18.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
-
TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation.
Authors: Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA
J. Immunol., 2008-09-15;181(6):4279-86.
Species: Mouse
Sample Types: BALF
-
Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
Authors: Wells JW, Cowled CJ, Farzaneh F
J. Immunol., 2008-09-01;181(5):3422-31.
Species: Mouse
Sample Types: Serum
-
Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
Biochem. Biophys. Res. Commun., 2008-08-13;375(2):275-9.
Species: Mouse
Sample Types: BALF
-
The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
Nat. Immunol., 2008-07-20;9(9):1047-54.
Species: Mouse
Sample Types: Cell Culture Supernates
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The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.
Authors: Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C
J. Endocrinol., 2008-06-18;198(3):581-9.
Species: Mouse
Sample Types: Tissue Homogenates
-
Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
Eur. J. Pharmacol., 2008-06-16;590(1):400-8.
Species: Mouse
Sample Types: Tissue Homogenates
-
CD47 deficiency protects mice from lipopolysaccharide-induced acute lung injury and Escherichia coli pneumonia.
Authors: Su X, Johansen M, Looney MR, Brown EJ, Matthay MA
J. Immunol., 2008-05-15;180(10):6947-53.
Species: Mouse
Sample Types: BALF
-
Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses.
Authors: Son A, Nakamura H, Okuyama H, Oka S, Yoshihara E, Liu W, Matsuo Y, Kondo N, Masutani H, Ishii Y, Iyoda T, Inaba K, Yodoi J
Eur. J. Immunol., 2008-05-01;38(5):1358-67.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP.
Authors: Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S
J. Immunol., 2008-03-15;180(6):3670-9.
Species: Mouse
Sample Types: Whole Cells
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A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
Mol. Ther., 2008-02-05;16(4):749-56.
Species: Mouse
Sample Types: Serum
-
CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis.
Authors: Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA
Am. J. Respir. Crit. Care Med., 2007-11-07;177(3):301-8.
Species: Mouse
Sample Types: Plasma
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Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
Blood, 2007-09-20;111(1):219-28.
Species: Mouse
Sample Types: Cell Culture Supernates
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Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
J. Immunol., 2007-09-15;179(6):3638-47.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Dectin-1 interaction with Mycobacterium tuberculosis leads to enhanced IL-12p40 production by splenic dendritic cells.
Authors: Rothfuchs AG, Bafica A, Feng CG, Egen JG, Williams DL, Brown GD, Sher A
J. Immunol., 2007-09-15;179(6):3463-71.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock.
Authors: Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J
Nat. Med., 2007-09-02;13(9):1042-9.
Species: Mouse
Sample Types: Serum
-
Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Authors: Lin YL, Liang YC, Chiang BL
J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
Species: Human
Sample Types: Cell Culture Supernates
-
IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
Authors: Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S
J. Immunol., 2007-06-15;178(12):7607-15.
Species: Mouse
Sample Types: Serum
-
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Authors: Fujita H, Asahina A, Komine M, Tamaki K
Cell. Immunol., 2007-05-15;245(2):70-9.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
Am. J. Pathol., 2007-04-01;170(4):1152-64.
Species: Mouse
Sample Types: Tissue Homogenates
-
The immunomodulation of endotoxin-induced acute lung injury by hesperidin in vivo and in vitro.
Authors: Yeh CC, Kao SJ, Lin CC, Wang SD, Liu CJ, Kao ST
Life Sci., 2007-02-03;80(20):1821-31.
Species: Mouse
Sample Types: BALF
-
Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
J. Immunol., 2007-01-01;178(1):496-502.
Species: Mouse
Sample Types: BALF
-
CD27 contributes to the early systemic immune response to Mycobacterium tuberculosis infection but does not affect outcome.
Authors: Wieland CW, Kerver ME, Florquin S, Nolte MA, Borst J, van Lier R, van Oers MH, van der Poll T
Int. Immunol., 2006-09-11;18(11):1531-9.
Species: Mouse
Sample Types: Tissue Homogenates
-
Adenosine A2A receptor inactivation increases survival in polymicrobial sepsis.
Authors: Nemeth ZH, Csoka B, Wilmanski J, Xu D, Lu Q, Ledent C, Deitch EA, Pacher P, Spolarics Z, Hasko G
J. Immunol., 2006-05-01;176(9):5616-26.
Species: Mouse
Sample Types: Plasma
-
Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
Eur. Respir. J., 2006-04-26;28(2):275-85.
Species: Mouse
Sample Types: BALF
-
MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo.
Authors: Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F
J. Immunol., 2005-11-15;175(10):6723-32.
Species: Mouse
Sample Types: Serum
-
Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
Vaccine, 2005-09-22;24(9):1470-7.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
J. Immunol., 2005-02-15;174(4):2327-35.
Species: Rat
Sample Types: Cell Culture Supernates
-
Endogenous interleukin-12 improves the early antimicrobial host response to murine Escherichia coli peritonitis.
Authors: Weijer S, Florquin S, van der Poll T
Shock, 2005-01-01;23(1):54-8.
Species: Mouse
Sample Types: Peritoneal Fluid
-
Vaccination of apoptotic Fas ligand-expressing tumors decreased antitumor responses by enhanced production of immunosuppressive cytokines.
Authors: Wada A, Tada Y, Shimozato O, Takiguchi Y, Tatsumi K, Kuriyama T, Tagawa M
Anticancer Res., 2005-01-01;25(1):299-303.
Species: Mouse
Sample Types: Tissue Secretion
-
Gamma interferon does not enhance clearance of Pseudomonas aeruginosa but does amplify a proinflammatory response in a murine model of postseptic immunosuppression.
Authors: Murphey ED, Herndon DN, Sherwood ER
Infect. Immun., 2004-12-01;72(12):6892-901.
Species: Mouse
Sample Types: Plasma
-
Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411.
Species: Mouse
Sample Types: Serum
-
Increased and long-term generation of dendritic cells with reduced function from IL-6-deficient bone marrow.
Authors: Bleier JI, Pillarisetty VG, Shah AB, DeMatteo RP
J. Immunol., 2004-06-15;172(12):7408-16.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
Med. Sci. Monit., 2004-03-01;10(3):BR77-83.
Species: Mouse
Sample Types: BALF
-
Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
J. Immunol., 2004-02-01;172(3):1754-62.
Species: Mouse
Sample Types: Cell Culture Supernates
-
A protective role of locally administered immunostimulatory CpG oligodeoxynucleotide in a mouse model of genital herpes infection.
Authors: Harandi AM, Eriksson K, Holmgren J
J. Virol., 2003-01-01;77(2):953-62.
Species: Mouse
Sample Types: Serum
-
Cutting edge: myeloid differentiation factor 88 deficiency improves resistance against sepsis caused by polymicrobial infection.
Authors: Weighardt H, Bê Ë„Æ%í, Kaiser-Moore S, Vabulas RM, Kirschning CJ, Wagner H, Holzmann B
2002-09-15;169(6):2823-7.
Species: Mouse
Sample Types: Serum
-
Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
Authors: Miller G, 107331, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1alpha and the NAMPT-NAD+ pathway, 2002-09-15;169(6):2875-85.
Species: Mouse
Sample Types: Serum
-
A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
Authors: Huaux F, 107322, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms, 2002-09-01;169(5):2653-61.
Species: Mouse
Sample Types: Tissue Homogenates
-
The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
Eur. J. Immunol., 2001-07-01;31(7):2104-15.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
Infect. Immun., 2000-06-01;68(6):3297-304.
Species: Rat
Sample Types: Vaginal Fluid
-
In vitro and in vivo immunostimulatory potential of bone marrow-derived mast cells on B- and T-lymphocyte activation.
Authors: Tkaczyk C, Villa I, Peronet R, David B, Chouaib S, Mecheri S
J. Allergy Clin. Immunol., 2000-01-01;105(1):134-42.
Species: Mouse
Sample Types: Cell Culture Supernates
FAQs
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Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
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The Mouse IL-12 p70 ELISA Kit is stable. The R2 value of the standard curve is more than 0.99. Easy to use!
Cell culture supernatants produced from transfected BHK-21 cells