Mouse IL-12 R beta 1 PE-conjugated Antibody Summary
Val32-Glu561
Accession # Q60837
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑12 R beta 1 in D10.G4.1 Mouse Cell Line by Flow Cytometry. D10.G4.1 mouse helper T cell line was stained with Goat Anti-Mouse IL-12 R beta 1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1998P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 1
IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%-21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4-6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8-10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).
- Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
- Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
- Chua, A.O. et al. (1995) J. Immunol. 155:4286.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Wu, C. et al. (1997) J. Immunol. 159:1658.
- Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
- Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
- Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
- Airoldi, I. et al. (2000) J. Immunol. 165:6880.
- Li, J. et al. (2003) J. Neurol. Sci. 215:95.
- Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
- Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
- Shimozato, O. et al. (2006) Immunology 117:22.
- Russell, T.D. et al. (2003) J. Immunol. 171:6866.
Product Datasheets
Citations for Mouse IL-12 R beta 1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Synthetic deletion of the Interleukin 23 receptor (IL-23R) stalk region led to autonomous IL-23R homodimerization and activation
Authors: TM Hummel, T Ackfeld, M Schönberg, G Ciupka, F Schulz, A Oberdoerst, J Grötzinger, J Scheller, DM Floss
Mol. Cell. Biol., 2017-08-11;0(0):.
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EBV-induced gene 3 augments IL-23R&alpha protein expression through a chaperone calnexin
Authors: I Mizoguchi, M Ohashi, H Hasegawa, Y Chiba, N Orii, S Inoue, C Kawana, M Xu, K Sudo, K Fujita, M Kuroda, SI Hashimoto, K Matsushima, T Yoshimoto
J. Clin. Invest., 2020-11-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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