Mouse IL-17 ELISpot Kit

Catalog #: EL421
8 Citations Datasheet / COA / SDS

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XEL421
EL421

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Citations (8)

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Summary Product Datasheets Preparation and Storage Background

Mouse IL-17 ELISpot Kit Summary

Assay Type

Quantitative Sandwich ELISA

Format

96-well PVDF-backed microplate

Assay Length

3 hours 15 mins to 4 hours 30 mins*

Sample Type

Whole Cells

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

PRODUCT SUMMARY

ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IL-17/IL-17A. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IL-17/IL-17A, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IL-17/IL-17A. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IL-17/IL-17A secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

PRODUCT FEATURES

Detect and quantitate individual cells secreting mouse IL-17/IL-17A
High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
No in vitro expansion of cells required
High-throughput - ELISpot assays use only a small number of primary cells

KIT CONTENTS

Mouse IL-17/IL-17A Microplate
Biotinylated Detection Antibody
Streptavidin conjugated to Alkaline Phosphatase
Dilution Buffers
Wash Buffer Concentrate
BCIP/NBT Chromogen
Mouse IL-17/IL-17A Positive Control

OTHER REAGENTS REQUIRED

Pipettes and pipette tips
Deionized or distilled water
Squirt bottle, manifold dispenser, or automated microplate washer
500 mL graduated cylinder
37 °C CO₂ incubator
Sterile culture media
Dissection microscope or an automated ELISpot reader

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:

Interleukin 17

Entrez Gene IDs:

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names:

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-17 ELISpot Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Scavenger receptor-A is a biomarker and effector of rheumatoid arthritis: A large-scale multicenter study
Authors: F Hu, X Jiang, C Guo, Y Li, S Chen, W Zhang, Y Du, P Wang, X Zheng, X Fang, X Li, J Song, Y Xie, F Huang, J Xue, M Bai, Y Jia, X Liu, L Ren, X Zhang, J Guo, H Pan, Y Su, H Yi, H Ye, D Zuo, J Li, H Wu, Y Wang, R Li, L Liu, XY Wang, Z Li
Nat Commun, 2020-04-20;11(1):1911.
Species: Mouse
Sample Types: Whole Cells
Broad and Effective Protection against Staphylococcus aureus Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
Authors: J Deng, X Wang, BZ Zhang, P Gao, Q Lin, RY Kao, K Gustafsson, KY Yuen, JD Huang
mSphere, 2019-09-04;4(5):.
Species: Mouse
Sample Types: Whole Cells
The increased protection and pathology in Mycobacterium tuberculosis-infected IL-27R-alpha-deficient mice is supported by IL-17A and is associated with the IL-17A-induced expansion of multifunctional T cells
Authors: H Erdmann, J Behrends, K Ritter, A Hölscher, J Volz, I Rosenkrand, C Hölscher
Mucosal Immunol, 2018-05-04;0(0):.
Species: Mouse
Sample Types: Whole Cells
Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
Authors: Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R
BMC Biol, 2014-05-09;12(0):32.
Species: Mouse
Sample Types: Cell Culture Supernates
Excretory/secretory products from Trichinella spiralis adult worms ameliorate DSS-induced colitis in mice.
Authors: Yang, Xiaodi, Yang, Yaping, Wang, Yunyun, Zhan, Bin, Gu, Yuan, Cheng, Yuli, Zhu, Xinping
PLoS ONE, 2014-05-02;9(5):e96454.
Species: Mouse
Sample Types: Cell Culture Supernates, Whole Cells
The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development.
Authors: Savino, Maria Te, Ulivieri, Cristina, Emmi, Giacomo, Prisco, Domenico, De Falco, Giulia, Ortensi, Barbara, Beccastrini, Enrico, Emmi, Lorenzo, Pelicci, Giuliana, D'Elios, Mario M, Baldari, Cosima T
J Leukoc Biol, 2013-01-23;93(4):549-59.
Species: Mouse
Sample Types: Whole Cells
TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
J. Immunol., 2008-09-15;181(6):4089-97.
Species: Mouse
Sample Types: Whole Cells
Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice.
Authors: Tan SL, Zhao J, Bi C, Chen XC, Hepburn DL, Wang J, Sedgwick JD, Chintalacharuvu SR, Na S
J. Immunol., 2006-03-01;176(5):2872-9.
Species: Mouse
Sample Types: Whole Cells