Mouse IL-17RB Antibody

Catalog #: AF1040
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1040
AF1040-SP

Detection of Mouse IL‑17 RB by Western Blot.

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All IL-17RB Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17RB Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑17 RB in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-17 R and recombinant human IL-17 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RB
Arg18-Gly286
Accession # Q9JIP3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse IL‑17 RB by Western Blot. Western blot shows lysates of mouse liver tissue and mouse spleen tissue (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1040) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17 RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL17B R cDNA encodes a 499 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17 RB also exists (4). Human and mouse IL‑17 RB share 76% aa sequence identity. The IL-17 RB protein sequence is only 19.2% and 18.2% identical to the human and mouse IL-17 R sequence, respectively. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17 RB is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17 RB is significantly up‑regulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2‑4).

Long Name

Interleukin 17B Receptor

Entrez Gene IDs

55540 (Human); 50905 (Mouse)

Alternate Names

CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor

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COA

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Citations for Mouse IL-17RB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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