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Mouse IL-17RC Antibody

Catalog #: AF2270
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2270
AF2270-SP
Detection of IL‑17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (6)
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Mouse IL-17RC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17 RC in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-17 RC is observed and less than 5% cross-reactivity with recombinant mouse (rm) IL‑17 RD and rmIL-17B R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RC
Leu21-Trp465
Accession # AAH04759
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑17 RC (Catalog # 2270-ML)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑17F-induced IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.6-3.6 µg/mL in the presence of 100 ng/mL Recombinant Human IL‑17F.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑17 RC antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL-17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Neutralization IL‑6 Secretion Induced by IL‑17F and Neutralization by Mouse IL‑17 RC Antibody. View Larger

IL‑6 Secretion Induced by IL‑17F and Neutralization by Mouse IL‑17 RC Antibody. Recombinant Human IL-17F (Catalog # 1335-INS) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secre-tion elicited by Recombinant Human IL-17F (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270). The ND50 is typically 0.6-3.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RC

IL-17 receptor C (IL-17 RC; also known as IL-17 RL) is an 85‑110 kDa member of the IL-17 receptor family. This is one of five families, termed IL-17 RA, B, C, D and E, that comprise the cytokine receptor superfamily (1‑6). Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17A, while IL-17 RB is reported to bind IL-17B and IL-17E (2, 4). Mouse IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. Full-length IL-17 RC is synthesized as a 674 amino acid (aa) precursor. It contains a 21 aa signal sequence, a 419 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 213 aa cytoplasmic region. There are multiple potential N‑linked glycosylation sites in the ECD and potential phosphorylation sites in the cytoplasmic tail. Four mouse variants have been identified that have been designated mIL-17 RC (7). The isoform expressed here as an R&D product is an unusual 567 aa form (8). Its precursor contains a 20 aa signal sequence, a 444 aa extracellular region, a 20 aa transmembrane segment and an 83 aa cytoplasmic tail. When compared to the full length mouse IL-17 RC form, this expressed isoform’s extracellular region shows absolute aa identity, save for an additional 24 aa insert. In the cytoplasmic region, it is highly divergent and shows virtually no aa identity (8‑9). The extracellular region of mouse IL-17 RC shows about 70% aa identity to the equivalent region in human IL-17 RC isoform # 3. IL-17 RC is the cognate receptor for IL-17F (7). In humans, IL-17 RC binds IL-17A with similar affinity, and with IL-17 RA, it forms a definitive receptor for both IL-17A and IL-17F (7). The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL-17 RA homodimer (4, 7, 10, 11). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

References
  1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
  2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
  5. You, Z. et al. (2006) Cancer Res. 66:175.
  6. You, Z. et al. (2007) Neoplasia 9:464.
  7. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  8. GenBank Accession # AAH04759.
  9. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  10. Toy, D. et al. (2006) J. Immunol. 177:36.
  11. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
Long Name
Interleukin 17 Receptor C, Transcript Variant 3
Entrez Gene IDs
84818 (Human); 171095 (Mouse)
Alternate Names
FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14

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Citations for Mouse IL-17RC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Pulmonary Regnase-1 orchestrates the interplay of epithelium and adaptive immune systems to protect against pneumonia
    Authors: Yoshinari Nakatsuka, Alexis Vandenbon, Takashi Mino, Masanori Yoshinaga, Takuya Uehata, Xiaotong Cui et al.
    Mucosal Immunology
  2. CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
    Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
    Nature Immunology, 2022-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
    Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
    Cell. Signal., 2020-06-15;73(0):109690.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Neutralization, Western Blot
  4. Respiratory syncytial virus infection of newborn CX3CR1-deficent mice induces a pathogenic pulmonary innate immune response
    Authors: S Das, M Raundhal, J Chen, TB Oriss, R Huff, JV Williams, A Ray, P Ray
    JCI Insight, 2017-09-07;2(17):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Th17 cells mediate clade-specific, serotype-independent mucosal immunity.
    Authors: Chen K, McAleer JP, Lin Y, Paterson DL, Zheng M, Alcorn JF, Weaver CT, Kolls JK
    Immunity, 2011-12-23;35(6):997-1009.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
    Authors: Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
    J. Immunol., 2009-06-01;182(11):7119-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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