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Mouse IL-18/IL-1F4 Antibody

Catalog #: D047-3
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
D047-3
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Citations (9)
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Mouse IL-18/IL-1F4 Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody reacts with mouse IL-18 on Immunoprecipitation and ELISA.
Source
Monoclonal Rat IgG2A Clone # 74
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse IL-18/IL-1F4
Formulation
100 μg IgG in 100 μL volume of PBS containing 50% glycerol, pH 7.2.  No preservative is contained.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunoprecipitation
5 μg/0.5 μg Mouse IL-18
 

Mouse IL-18/IL-1F4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑18/IL‑1F4 Biotinylated Antibody (Catalog # D048-6)

Standard: Recombinant Mouse IL-18/IL-1F4 Protein (Catalog # B002-5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody solution is stable for one year from the date of purchase when stored at -20 °C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Mouse IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism
    Authors: Li C, Zhang Y, Cheng X et al.
    Dev. Cell
  2. NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice
    Authors: Tran, LS;Ying, L;D'Costa, K;Wray-McCann, G;Kerr, G;Le, L;Allison, CC;Ferrand, J;Chaudhry, H;Emery, J;De Paoli, A;Colon, N;Creed, S;Kaparakis-Liaskos, M;Como, J;Dowling, JK;Johanesen, PA;Kufer, TA;Pedersen, JS;Mansell, A;Philpott, DJ;Elgass, KD;Abud, HE;Nachbur, U;Croker, BA;Masters, SL;Ferrero, RL;
    Nature communications
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Riboflavin, vitamin B2, attenuates NLRP3, NLRC4, AIM2, and non-canonical inflammasomes by the inhibition of caspase-1 activity
    Authors: H Ahn, GS Lee
    Sci Rep, 2020-11-05;10(1):19091.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  4. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017-02-16;12(2):e0171703.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  5. IL-18 expression results in a recombinant vaccinia virus that is highly attenuated and immunogenic.
    Authors: Verardi P, Legrand F, Chan K, Peng Y, Jones L, Yilma T
    J Interferon Cytokine Res, 2013-10-29;34(3):169-78.
    Species: Primate
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008-06-13;84(3):631-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  8. NLRP3 Inflammasome: Key Mediator of Neuroinflammation in Murine Japanese Encephalitis
    Authors: Deepak Kumar Kaushik, Malvika Gupta, Kanhaiya Lal Kumawat, Anirban Basu
    PLoS ONE
  9. Fructose-arginine, a non-saponin molecule of Korean Red Ginseng, attenuates AIM2 inflammasome activation
    Authors: Huijeong Ahn, Byung-Cheol Han, Seung-Ho Lee, Geun-Shik Lee
    Journal of Ginseng Research

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