Mouse IL-18 R alpha /IL-1 R5 Antibody

Catalog #: MAB12161
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12161-SP
MAB12161-100
MAB12161-500

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutraliza-tion by IL‑18 R alpha /IL‑1 R5 Antibody.

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-18 R alpha /IL-1 R5 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.

Source

Monoclonal Rat IgG_2A Clone # 112624

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha
Ser19-Gly326
Accession # Q61098

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑18 R alpha /IL‑1 R5

Neutralization

Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutraliza-tion by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 Ra/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND₅₀ is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Long Name

Interleukin 18 Receptor alpha

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Alternate Names

CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-18 R alpha /IL-1 R5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Innate acting memory Th1 cells modulate heterologous diseases
Authors: Rakebrandt, N;Yassini, N;Kolz, A;Schorer, M;Lambert, K;Goljat, E;Estrada Brull, A;Rauld, C;Balazs, Z;Krauthammer, M;Carballido, JM;Peters, A;Joller, N;
Proceedings of the National Academy of Sciences of the United States of America
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
Authors: Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G
Nat Med, 2015-06-22;21(7):820-6.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation
The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
J. Immunol., 2011-06-29;187(3):1333-46.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Protection of CD8+ T cells from activation-induced cell death by IL-18.
Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
J. Leukoc. Biol., 2007-03-30;82(1):142-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
Authors: Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA
J. Immunol., 2007-02-01;178(3):1948-59.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Protective effect of neutralizing anti-IL-18 alpha monoclonal antibody on a mouse model of acute graft-versus-host disease
Authors: XIAOCUI LI, CUIPING ZHANG, WEI CHEN, BIN PAN, FANYUN KONG, KUIYANG ZHENG et al.
Oncology Reports