Mouse IL-1 alpha /IL-1F1 Antibody Summary
ser6-ser161
Accession # Q62161
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Mouse IL‑1 alpha /IL‑1F1 Antibody. Recombinant Mouse IL-1a/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Polyclonal Antibody (Catalog # AB-400-NA). The ND50 is typically 0.04-0.2 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 alpha/IL-1F1
IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4 and IL-13.
Product Datasheets
Citations for Mouse IL-1 alpha /IL-1F1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence
Authors: HN Kim, J Xiong, RS MacLeod, S Iyer, Y Fujiwara, KM Cawley, L Han, Y He, JD Thostenson, E Ferreira, RL Jilka, D Zhou, M Almeida, CA O'Brien
JCI Insight, 2020-10-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
Sci Rep, 2016-01-14;6(0):19404.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Cutting Edge: A Natural Antisense Transcript, AS-IL1alpha, Controls Inducible Transcription of the Proinflammatory Cytokine IL-1alpha.
Authors: Chan J, Atianand M, Jiang Z, Carpenter S, Aiello D, Elling R, Fitzgerald K, Caffrey D
J Immunol, 2015-07-15;195(4):1359-63.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
Authors: Banks WA, Farr SA, La Scola ME
J. Pharmacol. Exp. Ther., 2001-11-01;299(2):536-41.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Neutrophil-Mediated Lung Injury Both via TLR2-Dependent Production of IL-1 alpha and IL-12 p40, and TLR2-Independent CARDS Toxin after Mycoplasma pneumoniae Infection in Mice
Authors: Shigeyuki Tamiya, Eisuke Yoshikawa, Monami Ogura, Etsushi Kuroda, Koichiro Suzuki, Yasuo Yoshioka
Microbiology Spectrum
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Antimycobacterial and Anti-inflammatory Mechanisms of Baicalin via Induced Autophagy in Macrophages Infected with Mycobacterium tuberculosis
Authors: Qingwen Zhang, Jinxia Sun, Yuli Wang, Weigang He, Lixin Wang, Yuejuan Zheng et al.
Frontiers in Microbiology
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