Mouse IL-2 R beta Antibody

Catalog #: MAB5891 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5891
MAB5891-SP

Detection of IL‑2 R beta in Mouse Splenocytes by Flow Cytometry.

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All IL-2 R beta Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-2 R beta Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL-2 R beta, recombinant mouse (rm) IL-2 R gamma, rhIL-15 R alpha, or rmIL-21 R is observed.

Source

Monoclonal Rat IgG_2A Clone # 130122

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-2 R beta
Ala27-Glu240
Accession # P16297

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑2 R beta in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with anti-mouse Integrin a2/CD49b APC-conjugated antibody and either (A) Rat Anti-Mouse IL-2 R beta Monoclonal Antibody (Catalog # MAB5891) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2 R beta

IL‑2 R beta (IL-2 Receptor beta ; designated CD122) is a type I transmembrane protein that associates with IL-2 R alpha and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. IL‑2 R beta and the common gamma chain can also associate with IL-15 R alpha. IL-2 R beta is expressed by T, B, NK cells and monocyte/macrophages.

Long Name

Interleukin 2 Receptor beta

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Alternate Names

CD122 antigen; CD122; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-15 R beta; IL-2 R beta; IL-2 receptor subunit beta; IL2R beta; IL-2R subunit beta; IL2RB; IL-2Rb; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; P70-75; p75