Mouse IL-20 Antibody Summary
Leu25-Leu176
Accession # Q9JKV9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑20 and Neutralization by Mouse IL‑20 Antibody. Recombinant Mouse IL‑20 (Catalog # 1204-ML) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 Ra and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1204). The ND50 is typically 0.2-0.6 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-20
Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL‑20 R beta. The second complex is composed of IL-22 R and IL‑20 R beta. Whereas the IL‑22 R/IL‑20 R beta complex is shared with IL-24/mda-7, the IL‑20 R alpha /IL‑20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1‑6).
- Blumberg, H. et al. (2001) Cell 104:9.
- Liu, L. et al. (2003) Blood 102:3206.
- Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Dumoutier, L. (2001) J. Immunol. 167:3545.
- Romer, J. (2003) J. Invest. Dermatol. 121:1306.
Product Datasheets
Citation for Mouse IL-20 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Murine astrocytes are responsive to the pro-inflammatory effects of IL-20
Authors: Amanda R. Burmeister, M. Brittany Johnson, Ian Marriott
Neuroscience Letters
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