Mouse IL-22BP APC-conjugated Antibody Summary
Thr19-Pro230
Accession # AAI24999
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑22BP in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse IL-22BP APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2376A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-22BP
Interleukin 22 Binding Protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the Type II Cytokine Receptor Family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1-4). IL-10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6-8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8-10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL-22-induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).
- Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
- Kotenko, S.V. (2002) Cytokine Growth Factor Rev. 13:223.
- Wolk, K. et al. (2005) Genes Immun. 6:8.
- Wei, C.C. et al. (2003) Genes Immun. 4:204.
- Dumoutier, L. et al. (2001) J. Immunol. 166:7090.
- Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
- Lecart, S. et al. (2002) Int. Immunol. 14:1351.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
Product Datasheets
Citation for Mouse IL-22BP APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expression
Authors: C Tang, H Sun, M Kadoki, W Han, X Ye, Y Makusheva, J Deng, B Feng, D Qiu, Y Tan, X Wang, Z Guo, C Huang, S Peng, M Chen, Y Adachi, N Ohno, S Trombetta, Y Iwakura
Nature Communications, 2023-03-17;14(1):1493.
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