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Mouse IL-24 Antibody

Catalog #: MAB2786
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2786
MAB2786-SP
IL‑24 in Mouse Splenocytes.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-24 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-24 in direct ELISAs and Western blots. No cross-reactivity with recombinant human IL-24 is observed.
Source
Monoclonal Rat IgG2A Clone # 303308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-24
Arg27-His181
Accession # NP_444325
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL-24
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-24 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

IL‑24 in Mouse Splenocytes. IL-24 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-24 Monoclonal Antibody (Catalog # MAB2786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-24

Mouse IL-24, also known as melanoma differentiation-associated gene 7 (MDA-7) and Th2-specific cytokine FISP, is a 27 kDa secreted glycoprotein that is a member of the IL-10 family. It is synthesized as a precursor with a large pro-region that is removed to generate a 155 aa mature protein. Mature mouse IL-24 shares 84% and 69% amino acid identity with rat and human IL-24, respectively.

Long Name
Interleukin 24
Entrez Gene IDs
11009 (Human); 93672 (Mouse); 170819 (Rat)
Alternate Names
C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

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Citations for Mouse IL-24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. IL-24 intrinsically regulates Th17 cell pathogenicity in mice
    Authors: Christopher Sie, Ravi Kant, Christian Peter, Andreas Muschaweckh, Monika Pfaller, Lucy Nirschl et al.
    Journal of Experimental Medicine
  2. IL-24 deficiency protects mice against bleomycin-induced pulmonary fibrosis by repressing IL-4-induced M2 program in macrophages
    Authors: LZ Rao, Y Wang, L Zhang, G Wu, L Zhang, FX Wang, LM Chen, F Sun, S Jia, S Zhang, Q Yu, JH Wei, HR Lei, T Yuan, J Li, X Huang, B Cheng, J Zhao, Y Xu, BW Mo, CY Wang, H Zhang
    Cell Death Differ, 2020-11-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats
    Authors: CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang
    Int J Mol Sci, 2019-09-18;20(18):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Murine astrocytes produce IL-24 and are susceptible to the immunosuppressive effects of this cytokine
    Authors: AR Burmeister, MB Johnson, JJ Yaemmongko, I Marriott
    J Neuroinflammation, 2019-03-02;16(1):55.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012-07-05;7(7):e40343.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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