Mouse IL-24 Biotinylated Antibody

Catalog #: BAM2786 Datasheet / COA / SDS

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BAM2786

All IL-24 Antibodies

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Mouse IL-24 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-24 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL‑24 is observed.

Source

Monoclonal Rat IgG_2A Clone # 303308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL‑24
Gln27-Leu181
Accession # Q925S4.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑24

Immunocytochemistry

8-25 µg/mL

Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-24

Mouse IL-24, also known as melanoma differentiation-associated gene 7 (MDA-7) and Th2-specific cytokine FISP, is a 27 kDa secreted glycoprotein that is a member of the IL-10 family. It is synthesized as a precursor with a large pro-region that is removed to generate a 155 aa mature protein. Mature mouse IL-24 shares 84% and 69% amino acid identity with rat and human IL-24, respectively.

Long Name

Interleukin 24

Entrez Gene IDs

11009 (Human); 93672 (Mouse); 170819 (Rat)

Alternate Names

C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein