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Mouse IL-27 Antibody

Catalog #: MAB18342
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB18342
MAB18342-SP
Detection of IL-27 in Mouse Splenocytes by Flow Cytometry.
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Citations (2)
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Mouse IL-27 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-27 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) IL‑27 p28, rmIL‑27 EBI3, recombinant human (rh) IL-27 p28, and rhIL-27 EBI3 is observed.
Source
Monoclonal Rat IgG2A Clone # 355025
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer
Tyr19-Pro228 (EBI3), Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-27 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL-27 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes untreated (open histogram) or treated with 1 µg/mL LPS for 24 hours (filled histogram) were stained with Mouse IL-27 Monoclonal Antibody (Catalog # MAB18342) or isotype control antibody (Catalog # MAB006, not shown), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27

Interleukin 27 (IL-27) is a secreted heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus‑induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naïve CD4+ T cells and induces the expression of a functional IL-12 receptor, allowing IL-12-induced Th1 polarization. Human EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.

Long Name
Interleukin 27
Entrez Gene IDs
246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)
Alternate Names
IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A

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Citations for Mouse IL-27 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IL-27-containing exosomes secreted by innate B-1a cells suppress and ameliorate uveitis
    Authors: Minkyung Kang, Manoj Kumar Yadav, Evaristus C. Mbanefo, Cheng-Rong Yu, Charles E. Egwuagu
    Frontiers in Immunology
  2. Lactococcus lactis Delivery of Surface Layer Protein A Protects Mice from Colitis by Re-Setting Host Immune Repertoire
    Authors: AP Arukha, CF Freguia, M Mishra, JK Jha, S Kariyawasa, NA Fanger, EM Zimmermann, GR Fanger, B Sahay
    Biomedicines, 2021-08-29;9(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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