Mouse IL-27 p28/IL-30 Antibody

Catalog #: MAB1834 Datasheet / COA / SDS

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MAB1834
MAB1834-SP

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Detection of IL‑27 in LPS-treated RAW 264.7 Mouse Cell Line by Flow Cytometry.

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Mouse IL-27 p28/IL-30 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.

Source

Monoclonal Rat IgG_2B Clone # 234205

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL-27 p28/IL-30 (Catalog # 7430-ML)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-27 antibody in LPS-treated RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

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Detection of IL‑27 in LPS-treated RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was unstimulated (light orange open histogram) or treated with 1 µg/mL LPS for 20 hours, then stained with Rat Anti-Mouse IL-27 p28/IL-30 Monoclonal Antibody (Catalog # MAB1834, dark orange filled histogram) or isotype control (Catalog # MAB0061, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 p28/IL-30

Interleukin 27 (IL-27) is a novel heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI-3 (Epstein‑Barr virus‑induced gene 3). IL-27 binds TCCR/WSX-1 on naïve CD4⁺ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL‑12‑mediated Th1 cell development.

Long Name

Interleukin 27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Alternate Names

IL27 p28; IL-27 p28; IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28; IL-30