Mouse IL-28B/IFN-lambda 3 Antibody

Catalog #: MAB1789
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1789
MAB1789-SP

IL‑28B/IFN‑ lambda 3 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑28B/IFN‑ lambda 3 Antibody.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-28B/IFN-lambda 3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑28B/IFN‑ lambda 3 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) IL-28A/IFN-gamma 2 and no cross-reactivity with recombinant human (rh) IL-28A or rhIL-29 is observed. This antibody neutralizes 100% of the bioactivity of rmIL-28A/IFN-gamma 2.

Source

Monoclonal Rat IgG_2B Clone # 244710

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL‑28B/IFN‑ lambda 3
Asp20-Val193
Accession # Q8CGK6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑28B/IFN-lambda 3 (Catalog # 1789-ML)

Neutralization

Measured by its ability to neutralize IL‑28B/IFN‑ lambda 3 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The Neutralization Dose (ND₅₀) is typically 1.5-6.0 µg/mL in the presence of 400 ng/mL Recombinant Mouse IL‑28B/IFN‑ lambda 3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IL‑28B/IFN‑ lambda 3 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑28B/IFN‑ lambda 3 Antibody. Recombinant Mouse IL-28B/ IFN-lambda 3 (Catalog # 1789-ML) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IL-28B/IFN-lambda 3 (400 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-28B/IFN-lambda 3 Monoclonal Antibody (Cata-log # MAB1789). The ND₅₀ is typically 1.5-6.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28B/IFN-lambda 3

Human IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% amino acid (aa) sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double‑stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up‑regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Mouse IL-28B cDNA encodes a 193 amino acid residue precursor protein with a putative 15 aa signal peptide. It shares 61%, 62% and 52% aa sequence identity with human IL-28A, human IL-28B and human IL-29, respectively.

References

Vilcek, J. (2003) Nature Immunol. 4:8.
Sheppard, P. et al. (2003) Nature Immunol. 4:63.
Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

Long Name

Interleukin 28B

Entrez Gene IDs

282617 (Human); 338374 (Mouse)

Alternate Names

interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3

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Citations for Mouse IL-28B/IFN-lambda 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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