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Mouse IL-33 Antibody

Catalog #: MAB3626
26 Citations 4 Reviews Datasheet / COA / SDS
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MAB3626
MAB3626-SP
Detection of Mouse IL‑33 by Western Blot.
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Product Details
Citations (26)
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Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-33 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-33 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 396118
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-33
Ser109-Ile266
Accession # Q8BVZ5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL-33 antibody by Western Blot. View Larger

Detection of Mouse IL‑33 by Western Blot. Western blot shows lysates of mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for IL-33 at approximately 18 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL‑33 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

IL‑33 in bEnd.3 Mouse Cell Line. IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel, Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of IL-33 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
 
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

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Citations for Mouse IL-33 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Interleukin 33 regulates gene expression in intestinal epithelial cells independently of its nuclear localization
    Authors: Z He, L Chen, GC Furtado, SA Lira
    Cytokine, 2018-08-23;111(0):146-153.
  2. Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
    Authors: Uapinyoying P, Hogarth M, Battacharya S et al.
    iScience
  3. Gata3 is detrimental to regulatory T cell function in autoimmune diabetes
    Authors: B Kiaf, K Bode, C Schuster, S Kissler
    bioRxiv : the preprint server for biology, 2023-03-23;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Loss of SLC26A3 Results in Colonic Mucosal Immune Dysregulation via Epithelial-Immune Cell Crosstalk
    Authors: D Jayawarden, S Priyamvada, T Kageyama, Z White, A Kumar, TF Griggs, A Majumder, R Akram, AN Anbazhagan, T Sano, PK Dudeja
    Cellular and Molecular Gastroenterology and Hepatology, 2022-12-17;0(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy
    Authors: Deepti Sharma, Shivantika Bisen, Geetika Kaur, Eric C. Van Buren, Gadiparthi N. Rao, Nikhlesh K. Singh
    Communications Biology
  6. Myeloid-IL4Ralpha is an indispensable link in IL-33-ILCs-IL-13-IL4Ralpha axis of eosinophil recruitment in murine lungs
    Authors: S Patial, BW Lewis, T Vo, I Choudhary, K Paudel, Y Mao, D Singamsett, F Brombacher, Y Saini
    Scientific Reports, 2021-07-29;11(1):15465.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption
    Authors: S Toyama, CS Moniaga, S Nakae, M Kurosawa, H Ogawa, M Tominaga, K Takamori
    International Journal of Molecular Sciences, 2021-07-12;22(14):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells
    Authors: F Kubo, DM Ariestanti, S Oki, T Fukuzawa, R Demizu, T Sato, RM Sabirin, S Hirose, N Nakamura
    Respir. Res., 2019-01-17;20(1):11.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels
    Authors: Mark Barbour, Rachel Wood, Shehla U. Hridi, Chelsey Wilson, Grant McKay, Trevor J. Bushell et al.
    Journal of Neuroimmunology
  10. Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection
    Authors: F Qi, S Bai, D Wang, L Xu, H Hu, S Zeng, R Chai, B Liu
    Mol. Immunol., 2017-05-19;87(0):284-292.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
    Authors: Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J
    J Immunol, 2015-09-04;195(8):3537-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration.
    Authors: Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams H, Lou Y
    J Immunol, 2015-01-23;194(5):2140-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
    Authors: Chen, Fei, Wu, Wenhui, Millman, Ariel, Craft, Joshua F, Chen, Eunice, Patel, Nirav, Boucher, Jean L, Urban, Joseph F, Kim, Charles, Gause, William
    Nat Immunol, 2014-08-31;15(10):938-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. The alarmin IL-33 promotes regulatory T-cell function in the intestine.
    Authors: Schiering C, Krausgruber T, Chomka A, Frohlich A, Adelmann K, Wohlfert E, Pott J, Griseri T, Bollrath J, Hegazy A, Harrison O, Owens B, Lohning M, Belkaid Y, Fallon P, Powrie F
    Nature, 2014-07-16;513(7519):564-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis.
    Authors: Carlock C, Wu J, Zhou C, Tatum K, Adams H, Tan F, Lou Y
    J Immunol, 2014-05-23;193(1):161-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. alpha-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
    Authors: Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, Wong C, Yu A
    J Immunol, 2014-01-24;192(4):1972-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014-01-15;7(5):1079-93.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  18. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    Immunology, 2013-06-01;139(2):205-18.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation.
    Authors: Juncadella, Ignacio, Kadl, Alexandr, Sharma, Ashish K, Shim, Yun M, Hochreiter-Hufford, Amelia, Borish, Larry, Ravichandran, Kodi S
    Nature, 2012-12-12;493(7433):547-51.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  20. Inducible IL-33 expression by mast cells is regulated by a calcium-dependent pathway.
    Authors: Hsu C, Bryce P
    J Immunol, 2012-08-24;189(7):3421-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. IL-33 attenuates EAE by suppressing IL-17 and IFN-gamma production and inducing alternatively activated macrophages.
    Authors: Jiang HR, Milovanovic M, Allan D, Niedbala W, Besnard AG, Fukada SY, Alves-Filho JC, Togbe D, Goodyear CS, Linington C, Xu D, Lukic ML, Liew FY
    Eur. J. Immunol., 2012-06-12;42(7):1804-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Production and functions of IL-33 in the central nervous system.
    Authors: Yasuoka S, Kawanokuchi J, Parajuli B, Jin S, Doi Y, Noda M, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A
    Brain Res., 2011-02-22;1385(0):8-17.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  23. IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
    Authors: Hsu CL, Neilsen CV, Bryce PJ
    PLoS ONE, 2010-08-03;5(8):e11944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008-06-13;84(3):631-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  25. Osteoblast Hypoxia-Inducible Factor-1a Pathway Activation Restrains Osteoclastogenesis via the Interleukin-33-MicroRNA-34a-Notch1 Pathway.
    Authors: Kang H, Yang K, Xiao L et al.
    Front Immunol.
  26. Interleukin-33 primes mast cells for activation by IgG immune complexes.
    Authors: Kaieda S, Wang J, Shnayder R, Fishgal N, Hei H, Lee R, Stevens R, Nigrovic P
    PLoS ONE, 2012-10-11;7(10):e47252.

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Reviews for Mouse IL-33 Antibody

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Mouse IL-33 Antibody
By Anonymous on 02/17/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Retinal Pigment Epithelium cells Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse IL-33 Antibody MAB3626
Mouse IL-33 Antibody
By Anonymous on 09/29/2021
Application: IHC Sample Tested: Lung tissue Species: Mouse
Immunohistochemistry Mouse IL-33 Antibody MAB3626
Mouse IL-33 Antibody
By Anonymous on 07/22/2016
Application: WB Sample Tested: Cell Lysates Species: Mouse
Mouse IL‑33 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Sputum

Loaded 5mcg protein/lane. Primary incubated O/N at 4 degrees with rocking.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: TBS-Tween, 5% non-fat milk
Dilution: 1:1000

Western Blot Mouse IL‑33 Antibody MAB3626