Mouse IL-33 PE-conjugated Antibody Summary
Ser109-Ile266
Accession # Q8BVZ5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 PE-conjugated Mono-clonal Antibody (Catalog # IC3626P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-33
IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.
- Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
- Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
- Schmitz, J. et al. (2005) Immunity 23:479.
- Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
- Xu, D. et al. (1998) J. Exp. Med. 187:787.
- Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
- Dinarello, C.A. (2005) Immunity 23:461.
- Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Product Datasheets
Citations for Mouse IL-33 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms
Authors: M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier
Mucosal Immunol, 2017-12-20;11(3):615-626.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis
Authors: S Chen, B Chen, Z Wen, Z Huang, L Ye
Oncotarget, 2017-05-16;8(20):32407-32418.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
Authors: Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A
PLoS ONE, 2015-08-14;10(8):e0135776.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33.
Authors: Verma, A, Kroetz, D N, Tweedle, J L, Deepe, G S Jr
Mucosal Immunol, 2014-08-13;8(2):380-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
Mucosal Immunol, 2014-01-15;7(5):1079-93.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Murine mast cells secrete and respond to interleukin-33.
Authors: Tung, Hui-Ying, Plunkett, Beverly, Huang, Shau-Ku, Zhou, Yufeng
J Interferon Cytokine Res, 2013-09-12;34(3):141-7.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
Authors: Mizutani N, Nabe T, Yoshino S
Immunology, 2013-06-01;139(2):205-18.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection.
Authors: Wills-Karp M, Rani R, Dienger K
J. Exp. Med., 2012-02-13;209(3):607-22.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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Cells were activated with PMA and ionomycin for 2 hrs before intracellular staining.
Mast cells were cultured from the bone marrow of IL-33-deficient or WT C57BL/6 mice. Genotypes were confirmed by PCR. Cells (5x10^5) were stained for viability, FceRI and c-kit, blocking Fc receptors, then fixed with BD Cytofix/Cytoperm. Cells were permeabilized with BD Permeabilization Buffer, and stained with 2ul anti-mouse IL-33-PE or rat IgG2a-PE isotype control. Cells were washed three times, and data were acquired on a BD FACSCanto LSR flow cytometer, gating on live c-Kit+FceRI+ cells.
Fluorescence increases by two orders of magnitude in both WT and IL-33-KO cells following staining with the IL-33-PE antibody relative to the isotype control. I am currently investigating whether there is any specific signal after stimulation of the cells, at which point IL-33 is induced. Staining with the recommended (greater) amount of antibody produces an even greater background shift in unstimulated cells.
R&D Systems Technical Service is investigating.
