Mouse IL-36Ra/IL-1F5 Antibody

Catalog #: MAB2714 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2714
MAB2714-SP

Detection of Mouse IL‑36Ra/IL‑1F5 by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-36Ra/IL-1F5 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-36Ra/IL-1F5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human IL-36Ra and recombinant mouse IL-1ra is observed.

Source

Monoclonal Rat IgG_2B Clone # 759207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL-36Ra/IL-1F5
Met1-Cys155
Accession # Q9QYY1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse IL‑36Ra/IL‑1F5 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-36Ra/IL-1F5 Monoclonal Antibody (Catalog # MAB2714) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-36Ra/IL-1F5 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36Ra/IL-1F5

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). Indirect evidence suggests the receptor for IL-36Ra/IL-1F5 is IL‑1 Rrp2 and/or IL-1 RAcP (9). IL-36Ra/IL-1F5 was initially reported to be an antagonist of IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

References

Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.
Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
Dunn, E. et al. (2001) Trends Immunol. 22:533.
Debets, R. et al. (2001) J. Immunol. 167:1440.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5

Entrez Gene IDs

26525 (Human); 54450 (Mouse)

Alternate Names

FIL1 delta; FIL1; FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta); FIL1DIL-1 delta; IL1F5 (Canonical product IL-1F5a); IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; interleukin 1 family, member 5 (delta); interleukin 1, delta; Interleukin 36 Receptor Antagonist; interleukin-1 family member 5; Interleukin-1 HY1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; Interleukin-36 Receptor Antagonist