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Mouse IL-5 ELISA Kit - Quantikine

Catalog #: M5000
92 Citations 3 Reviews Datasheet / COA / SDS
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Mouse IL-5 ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-5 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
7 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-5 in cell culture supernates and mouse serum. It contains Sf 21-expressed recombinant mouse IL-5 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse IL-5. Results obtained using natural mouse IL-5 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-5.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 41.5 117 428 43.4 123 447
Standard Deviation 2.4 3.9 8.6 1.8 5.3 20.8
CV% 5.8 3.3 2 4.1 4.3 4.7

Recovery

The recovery of mouse IL-5 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 99 85-110
Serum (n=5) 92 81-104

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-5 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-5 ELISA Linearity

Scientific Data

N/A Mouse IL-5 ELISA Standard Curve View Larger

Mouse IL-5 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Long Name:
Interleukin 5
Entrez Gene IDs:
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names:
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-5 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

92 Citations: Showing 1 - 10
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  1. Helminth alleviates COVID-19-related cytokine storm in an IL-9-dependent way
    Authors: Cao, Z;Wang, J;Liu, X;Liu, Y;Li, F;Liu, M;Chiu, S;Jin, X;
    mBio
    Species: Mouse
    Sample Types: Serum
  2. Fallopia japonica Root Extract Ameliorates Ovalbumin-Induced Airway Inflammation in a CARAS Mouse Model by Modulating the IL-33/TSLP/NF-?B Signaling Pathway
    Authors: Jin, J;Fan, YJ;Nguyen, TV;Yu, ZN;Song, CH;Lee, SY;Shin, HS;Chai, OH;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: BALF, NLF
  3. Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s
    Authors: Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K;
    Cell reports
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates, BALF
  4. Gene expression profiles and bioinformatics analysis in lung samples from ovalbumin-induced asthmatic mice
    Authors: Y Song, J Jiang, Q Bai, S Liu, Y Zhang, C Xu, H Piao, L Li, G Yan
    BMC pulmonary medicine, 2023-02-02;23(1):50.
    Species: Mouse
    Sample Types: BALF
  5. Physiological microbial exposure transiently inhibits mouse lung ILC2 responses to allergens
    Authors: KE Block, K Iijima, MJ Pierson, DA Walsh, R Tei, TA Kucaba, J Xu, MH Khan, C Staley, TS Griffith, HJ McSorley, H Kita, SC Jameson
    Nature Immunology, 2022-11-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Downregulation of deubiquitinating enzyme USP25 promotes the development of allergic rhinitis by enhancing TSLP signaling in the nasal epithelium
    Authors: W Chang, H Lv, L Tan, Z Gao, P Liu, D Qin, W Zhang, Y Xu
    Molecular Medicine Reports, 2022-09-30;26(5):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Serum
  7. Cinnamomum cassia (L.) J.Presl Alleviates Allergic Responses in Asthmatic Mice via Suppression of MAPKs and MMP-9
    Authors: JO Lim, YH Kim, IS Lee, WI Kim, SJ Lee, SW Pak, IS Shin, T Kim
    Frontiers in Pharmacology, 2022-08-11;13(0):906916.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Psychological Stress Exacerbates Inflammation of the Ileum via the Corticotropin-Releasing Hormone-Mast Cell Axis in a Mouse Model of Eosinophilic Enteritis
    Authors: A Kanamori, F Tanaka, M Ominami, Y Nadatani, S Fukunaga, K Otani, S Hosomi, N Kamata, Y Nagami, K Taira, Y Fujiwara
    International Journal of Molecular Sciences, 2022-08-01;23(15):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Immunoregulatory effects of Lactococcus lactis-derived extracellular vesicles in allergic asthma
    Authors: DH Lee, HK Park, HR Lee, H Sohn, S Sim, HJ Park, YS Shin, YK Kim, Y Choi, HS Park
    Clinical and translational allergy, 2022-03-01;12(3):e12138.
    Species: Mouse
    Sample Types: BALF
  10. Acteoside exerts immunomodulatory effects on dendritic cells via aryl hydrocarbon receptor activation and ameliorates Th2-mediated allergic asthma by inducing Foxp3+ regulatory T cells
    Authors: JH Chang, HC Chuang, G Hsiao, TY Hou, CC Wang, SC Huang, BY Li, YL Lee
    International immunopharmacology, 2022-02-03;106(0):108603.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Glutaminolysis is required in maintaining immune regulatory functions in B cells
    Authors: JQ Liu, XR Geng, TY Hu, LH Mo, XQ Luo, SY Qiu, DB Liu, ZG Liu, JB Shao, ZQ Liu, PC Yang
    Mucosal Immunology, 2022-01-10;0(0):.
    Species: Mouse
    Sample Types: Nasal Secretion
  12. Eosinophil extracellular traps drive asthma progression through neuro-immune signals
    Authors: Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su
    Nature Cell Biology, 2021-10-06;23(10):1060-1072.
    Species: Mouse
    Sample Types: BALF
  13. Arf6 exacerbates allergic asthma through cell-to-cell transmission of ASC inflammasomes
    Authors: S Lee, A Ishitsuka, T Kuroki, YH Lin, A Shibuya, T Hongu, Y Funakoshi, Y Kanaho, K Nagata, A Kawaguchi
    JCI Insight, 2021-08-23;6(16):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Isoalantolactone alleviates ovalbumin?induced asthmatic inflammation by reducing alternatively activated macrophage and STAT6/PPAR?gamma/KLF4 signals
    Authors: Y Song, X Li, F Liu, H Zhu, Y Shen
    Molecular Medicine Reports, 2021-08-09;24(4):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. HDAC4 induces the development of asthma by increasing Slug-upregulated CXCL12 expression through KLF5 deacetylation
    Authors: W Wei, W Chen, N He
    Journal of Translational Medicine, 2021-06-12;19(1):258.
    Species: Mouse
    Sample Types: BALF
  16. WLS/wntless is essential in controlling dendritic cell homeostasis via a WNT signaling-independent mechanism
    Authors: LT Wang, MH Lin, KY Liu, SS Chiou, SN Wang, CY Chai, LW Tseng, HC Chiou, HC Wang, KK Yokoyama, SH Hsu, SK Huang
    Autophagy, 2021-04-14;0(0):1-16.
    Species: Mouse
    Sample Types: BALF
  17. Structure-based design of a Cortistatin analogue with immunomodulatory activity in models of inflammatory bowel disease
    Authors: Á Rol, T Todorovski, P Martin-Mal, A Escolà, E Gonzalez-R, E Aragón, X Verdaguer, M Vallès-Mir, J Farrera-Si, E Puig, J Fernández-, B Ponsati, M Delgado, A Riera, MJ Macias
    Nature Communications, 2021-03-25;12(1):1869.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Alleviation of Ulcerative Colitis Potentially through th1/th2 Cytokine Balance by a Mixture of Rg3-enriched Korean Red Ginseng Extract and Persicaria tinctoria
    Authors: E Saba, YY Lee, MH Rhee, SD Kim
    Molecules, 2020-11-10;25(22):.
    Species: Mouse
    Sample Types: Plasma
  19. COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice
    Authors: W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles
    J. Immunol., 2020-07-20;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Innate Lymphoid Cells in the Induction of Obesity
    Authors: T Sasaki, K Moro, T Kubota, N Kubota, T Kato, H Ohno, S Nakae, H Saito, S Koyasu
    Cell Rep, 2019-07-02;28(1):202-217.e7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Effect of Lactobacillus rhamnosus GG immunopathologic changes in chronic mouse asthma model
    Authors: CT Wu, FH Lin, YT Lee, MS Ku, KH Lue
    J Microbiol Immunol Infect, 2019-03-23;0(0):.
    Species: Mouse
    Sample Types: BALF
  22. Andrographolide prevented toluene diisocyanate-induced occupational asthma and aberrant airway E-cadherin distribution via p38 MAPK-dependent Nrf2 induction
    Authors: I Sulaiman, K Tan, N Mohtarrudi, JC Woei Lim, J Stanslas
    Pulm Pharmacol Ther, 2018-09-21;0(0):.
    Species: Mouse
    Sample Types: BALF
  23. Accelerated inflammation and oxidative stress induced by LPS in acute lung injury: ?nhibition by ST1926
    Authors: Z Dong, Y Yuan
    Int. J. Mol. Med., 2018-03-19;0(0):.
    Species: Mouse
    Sample Types: Serum
  24. ROR?-expressing T regulatory cells restrain allergic skin inflammation
    Authors: N Malhotra, JM Leyva-Cast, U Jadhav, O Barreiro, C Kam, NK O'Neill, F Meylan, P Chambon, UH von Andria, RM Siegel, EC Wang, R Shivdasani, RS Geha
    Sci Immunol, 2018-03-02;3(21):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis
    Authors: C Zhang, W Yi, F Li, X Du, H Wang, P Wu, C Peng, M Luo, W Hua, CC Wong, JJ Lee, W Li, Z Chen, S Ying, Z Ju, H Shen
    Cell Res., 2018-01-12;0(0):.
    Species: Mouse
    Sample Types: Serum
  26. Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms
    Authors: M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier
    Mucosal Immunol, 2017-12-20;11(3):615-626.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017-06-29;0(0):.
    Species: Mouse
    Sample Types: BALF
  28. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Bach2 Controls Homeostasis of Eosinophils by Restricting the Type-2 Helper Function of T Cells
    Authors: Y Sato, H Kato, R Ebina-Shib, A Itoh-Nakad, R Okuyama, K Igarashi
    Tohoku J. Exp. Med, 2017-03-01;241(3):175-182.
    Species: Mouse
    Sample Types: Serum
  30. Justicia procumbens Extract (DW2008) Selectively Suppresses Th2 Cytokines in Splenocytes and Ameliorates Ovalbumin-Induced Airway Inflammation in a Mouse Model of Asthma
    Authors: J Youm, H Lee, HB Chang, J Jeon, MH Yoon, JY Woo, MS Choi, Y Hwang, S Seong, K Na, J Yoon
    Biol. Pharm. Bull., 2017-01-01;40(9):1416-1422.
    Species: Mouse
    Sample Types: BALF
  31. Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model�of chronic asthma
    Allergol Int, 2016-11-19;0(0):.
    Species: Mouse
    Sample Types: Complex Sample Type
  32. G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10.
    Authors: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M
    PLoS ONE, 2015-03-31;10(3):e0123210.
    Species: Mouse
    Sample Types: BALF
  33. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015-02-18;6(0):6224.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
    Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
    PLoS Pathog, 2015-02-06;11(2):e1004607.
    Species: Mouse
    Sample Types: Serum
  35. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling.
    Authors: Zhou W, Goleniewska K, Zhang J, Dulek D, Toki S, Lotz M, Newcomb D, Boswell M, Polosukhin V, Milne G, Wu P, Moore M, FitzGerald G, Peebles R
    J Allergy Clin Immunol, 2014-07-18;134(3):698-705.e5.
    Species: Mouse
    Sample Types: BALF
  36. Airway uric acid is a sensor of inhaled protease allergens and initiates type 2 immune responses in respiratory mucosa.
    Authors: Hara K, Iijima K, Elias M, Seno S, Tojima I, Kobayashi T, Kephart G, Kurabayashi M, Kita H
    J Immunol, 2014-03-24;192(9):4032-42.
    Species: Mouse
    Sample Types: BALF
  37. Eosinophils regulate peripheral B cell numbers in both mice and humans.
    Authors: Wong, Tina W, Doyle, Alfred D, Lee, James J, Jelinek, Diane F
    J Immunol, 2014-03-10;192(8):3548-58.
    Species: Mouse
    Sample Types: Serum
  38. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    Immunology, 2013-06-01;139(2):205-18.
    Species: Mouse
    Sample Types: Tissue Homogenates
  39. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011-12-23;188(3):1503-13.
    Species: Mouse
    Sample Types: BALF
  40. IL-33-activated dendritic cells are critical for allergic airway inflammation.
    Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
    Eur. J. Immunol., 2011-05-25;41(6):1675-86.
    Species: Mouse
    Sample Types: BALF
  41. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2011-05-10;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  42. Over-expression of the LTC4 synthase gene in mice reproduces human aspirin-induced asthma.
    Authors: Hirata H, Arima M, Fukushima Y, Honda K, Sugiyama K, Tokuhisa T, Fukuda T
    Clin. Exp. Allergy, 2011-03-24;41(8):1133-42.
    Species: Mouse
    Sample Types: BALF
  43. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
    Authors: Louten J, Rankin AL, Li Y
    Int. Immunol., 2011-03-21;23(5):307-15.
    Species: Mouse
    Sample Types: BALF
  44. Control of mouse graft-versus-host disease following allogeneic bone marrow transplantation by blocking the CD28/B7 signaling pathway with lentiviral vector-mediated RNA interference.
    Authors: Sang W, Zhou C, Cheng N, Li Z, Zeng L, Xu K
    Immunol. Lett., 2011-01-26;136(2):194-202.
    Species: Mouse
    Sample Types: Plasma
  45. Epithelial reticulon 4B (Nogo-B) is an endogenous regulator of Th2-driven lung inflammation.
    Authors: Wright PL, Yu J, Di YP
    J. Exp. Med., 2010-10-25;207(12):2595-607.
    Species: Mouse
    Sample Types: BALF
  46. Complement C3a regulates late asthmatic response and airway hyperresponsiveness in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    J. Immunol., 2009-08-14;183(6):4039-46.
    Species: Mouse
    Sample Types: BALF
  47. A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice.
    Authors: Page K, Ledford JR, Zhou P, Wills-Karp M
    J. Immunol., 2009-08-10;183(5):3400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  48. Peroxisome proliferator-activated receptor gamma agonist down-regulates IL-17 expression in a murine model of allergic airway inflammation.
    Authors: Park SJ, Lee KS, Kim SR, Min KH, Choe YH, Moon H, Chae HJ, Yoo WH, Lee YC
    J. Immunol., 2009-07-29;183(5):3259-67.
    Species: Mouse
    Sample Types: BALF
  49. Eosinophil viability is increased by acidic pH in a cAMP- and GPR65-dependent manner.
    Authors: Kottyan LC, Collier AR, Cao KH, Niese KA, Hedgebeth M, Radu CG, Witte ON, Khurana Hershey GK, Rothenberg ME, Zimmermann N
    Blood, 2009-07-29;114(13):2774-82.
    Species: Mouse
    Sample Types: Cell Lysates
  50. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
    Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
    J. Immunol., 2009-06-19;183(2):1291-300.
    Species: Mouse
    Sample Types: BALF
  51. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
    Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
    Inflamm. Res., 2009-06-09;58(12):845-54.
    Species: Mouse
    Sample Types: BALF
  52. Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
    Authors: Park SK, Cho MK, Park HK, Lee KH, Lee SJ, Choi SH, Ock MS, Jeong HJ, Lee MH, Yu HS
    J. Immunol., 2009-06-01;182(11):6907-14.
    Species: Mouse
    Sample Types: BALF
  53. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
    Authors: Kim DY, Park JW, Jeoung D, Ro JY
    Eur. J. Pharmacol., 2009-04-05;612(1):98-105.
    Species: Mouse
    Sample Types: BALF
  54. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009-04-03;27(23):3045-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  55. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
    Authors: Shinagawa K, Ploplis VA, Castellino FJ
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-03-13;296(5):L763-70.
    Species: Mouse
    Sample Types: BALF
  56. Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis.
    Authors: Palmer G, Talabot-Ayer D, Lamacchia C, Toy D, Seemayer CA, Viatte S, Finckh A, Smith DE, Gabay C
    Arthritis Rheum., 2009-03-01;60(3):738-49.
    Species: Mouse
    Sample Types: BALF
  57. Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7.
    Authors: Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P
    J. Immunol., 2009-01-15;182(2):880-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses.
    Authors: Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS
    J. Immunol., 2008-10-15;181(8):5360-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  59. T-bet expression by dendritic cells is required for the repolarization of allergic airway inflammation.
    Authors: Heckman KL, Radhakrishnan S, Peikert T, Iijima K, McGregor HC, Bell MP, Kita H, Pease LR
    Eur. J. Immunol., 2008-09-01;38(9):2464-74.
    Species: Mouse
    Sample Types: BALF
  60. Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
    Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
    Biochem. Biophys. Res. Commun., 2008-08-13;375(2):275-9.
    Species: Mouse
    Sample Types: BALF
  61. GS143, an IkappaB ubiquitination inhibitor, inhibits allergic airway inflammation in mice.
    Authors: Hirose K, Wakashin H, Oki M, Kagami S, Suto A, Ikeda K, Watanabe N, Iwamoto I, Furuichi Y, Nakajima H
    Biochem. Biophys. Res. Commun., 2008-07-24;374(3):507-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
    Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
    J. Immunol., 2008-07-15;181(2):1491-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008-06-16;590(1):400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
    Authors: Johnson TR, Rothenberg ME, Graham BS
    J. Leukoc. Biol., 2008-06-02;84(3):748-59.
    Species: Mouse
    Sample Types: Tissue Homogenates
  65. Eosinophil-nerve interactions and neuronal plasticity in rat gut associated lymphoid tissue (GALT) in response to enteric parasitism.
    Authors: O'Brien LM, Fitzpatrick E, Baird AW, Campion DP
    J. Neuroimmunol., 2008-05-20;197(1):1-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  66. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008-03-03;205(3):699-710.
    Species: Mouse
    Sample Types: BALF
  67. Liposomal retinoic acids modulate asthma manifestations in mice.
    Authors: Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M
    J. Nutr., 2007-12-01;137(12):2730-6.
    Species: Mouse
    Sample Types: BALF
  68. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells.
    Authors: Radinger M, Bossios A, Alm AS, Jeurink P, Lu Y, Malmhall C, Sjostrand M, Lotvall J
    Allergy, 2007-12-01;62(12):1410-8.
    Species: Mouse
    Sample Types: BALF
  69. IL-27 suppresses Th2 cell development and Th2 cytokines production from polarized Th2 cells: a novel therapeutic way for Th2-mediated allergic inflammation.
    Authors: Yoshimoto T, Yoshimoto T, Yasuda K, Mizuguchi J, Nakanishi K
    J. Immunol., 2007-10-01;179(7):4415-23.
    Species: Mouse
    Sample Types: Serum
  70. Chronic intranasal administration of mould spores or extracts to unsensitized mice leads to lung allergic inflammation, hyper-reactivity and remodelling.
    Authors: Denis O, van den Brule S, Heymans J, Havaux X, Rochard C, Huaux F, Huygen K
    Immunology, 2007-05-16;122(2):268-78.
    Species: Mouse
    Sample Types: BALF
  71. FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions.
    Authors: Daniel C, Sartory NA, Zahn N
    Mol. Immunol., 2007-05-01;44(13):3305-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Prolonged inhaled allergen exposure can induce persistent tolerance.
    Authors: Van Hove CL, Maes T, Joos GF, Tournoy KG
    Am. J. Respir. Cell Mol. Biol., 2007-01-11;36(5):573-84.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  73. Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
    Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
    J. Immunol., 2007-01-01;178(1):496-502.
    Species: Mouse
    Sample Types: BALF
  74. Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation.
    Authors: Kitamura K, Takeda K, Koya T, Miyahara N, Kodama T, Dakhama A, Takai T, Hirano A, Tanimoto M, Harada M, Gelfand EW
    J. Immunol., 2007-01-01;178(1):480-8.
    Species: Mouse
    Sample Types: BALF
  75. D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma.
    Authors: Lee JS, Lee CM, Jeong YI, Jung ID, Kim BH, Seong EY, Kim JI, Choi IW, Chung HY, Park YM
    FEBS Lett., 2006-12-11;581(1):57-64.
    Species: Mouse
    Sample Types: BALF
  76. Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
    Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
    J. Allergy Clin. Immunol., 2006-10-23;119(1):123-31.
    Species: Mouse
    Sample Types: BALF
  77. Oral administration of an immunostimulatory DNA sequence from Bifidobacterium longum improves Th1/Th2 balance in a murine model.
    Authors: Takahashi N, Kitazawa H, Iwabuchi N, Xiao JZ, Miyaji K, Iwatsuki K, Saito T
    Biosci. Biotechnol. Biochem., 2006-08-01;70(8):2013-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
    Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
    J. Immunol., 2006-02-15;176(4):2568-80.
    Species: Mouse
    Sample Types: Serum
  79. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
    Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
    Life Sci., 2005-10-17;78(9):987-94.
    Species: Mouse
    Sample Types: BALF
  80. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005-09-22;24(9):1470-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  81. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005-08-15;175(4):2589-97.
    Species: Mouse
    Sample Types: BALF
  82. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005-02-15;174(4):2327-35.
    Species: Rat
    Sample Types: Cell Culture Supernates
  83. Formulation with CpG oligodeoxynucleotides prevents induction of pulmonary immunopathology following priming with formalin-inactivated or commercial killed bovine respiratory syncytial virus vaccine.
    Authors: Oumouna M, Mapletoft JW, Karvonen BC, Babiuk LA
    J. Virol., 2005-02-01;79(4):2024-32.
    Species: Mouse
    Sample Types: BALF
  84. MyD88-dependent induction of allergic Th2 responses to intranasal antigen.
    Authors: Piggott DA, Eisenbarth SC, Xu L, Constant SL, Huleatt JW, Herrick CA, Bottomly K
    J. Clin. Invest., 2005-02-01;115(2):459-67.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411.
    Species: Mouse
    Sample Types: Serum
  86. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
    Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
    J. Virol., 2004-08-01;78(16):8753-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
  87. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
    Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
    Med. Sci. Monit., 2004-03-01;10(3):BR77-83.
    Species: Mouse
    Sample Types: BALF
  88. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003-06-01;170(11):5756-63.
    Species: Mouse
    Sample Types: BALF
  89. Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.
    Authors: Drouin SM, Corry DB, Hollman TJ, Kildsgaard J, Wetsel RA
    J. Immunol., 2002-11-15;169(10):5926-33.
    Species: Mouse
    Sample Types: BALF
  90. Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide.
    Authors: Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A
    J. Exp. Med., 2001-09-17;194(6):833-46.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  91. Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
    Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
    Infect. Immun., 2000-06-01;68(6):3297-304.
    Species: Rat
    Sample Types: Vaginal Fluid
  92. Pulmonary eosinophilia and production of MIP-1alpha are prominent responses to infection with pneumonia virus of mice.
    Authors: Domachowske JB, Bonville CA, Dyer KD, Easton AJ, Rosenberg HF
    Cell. Immunol., 2000-03-15;200(2):98-104.
    Species: Mouse
    Sample Types: BALF

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Mouse IL-5 Quantikine ELISA Kit
By Anonymous on 12/31/2020
Sample Tested: Mouse peritoneal wash

The C57BL mice was I.P. challenged by gram-negative bacteria Acinetobacter baumannii and the peritoneal wash was collected at 5 hours post infection. The IL-5 was measured by ELISA.

Mouse IL-5 Quantikine ELISA Kit M5000
Mouse IL-5 Quantikine ELISA Kit
By Anonymous on 08/06/2018
Sample Tested: Serum
Mouse IL-5 Quantikine ELISA Kit
By Anonymous on 05/02/2017
Sample Tested: Lung tissue,RAW 264.7 mouse monocyte/macrophage cell line