Mouse IL-6R alpha Biotinylated Antibody

Catalog #: BAF1830
4 Citations Datasheet / COA / SDS

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BAF1830

All IL-6R alpha Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-6R alpha Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-6R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant human IL‑6R, recombinant mouse (rm) IL‑6, and rmgp130 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-6R alpha
Leu20-Glu357
Accession # P22272

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL-6R alpha (Catalog # 1830-SR)

Flow Cytometry

2.5 µg/10⁶ cells

Mouse CD3⁺ splenocytes

Mouse IL-6R alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IL-6R alpha Antibody (Catalog # AF1830)

Standard: Recombinant Mouse IL-6R alpha (aa 20-357) Protein (Catalog # 1830-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51%, and 50% aa identity with rat, human, and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

References

Yamasaki, K. et al. (1988) Science 241:825.
Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
Hibi, M. et al. (1990) Cell 63:1149.
Saito, M. et al. (1992) J. Immunol. 148:4066.
Novick, D. et al. (1989) J. Exp. Med. 170:1409.
Jones, S.A. et al. (2001) FASEB J. 15:43.
Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Long Name

Interleukin 6 Receptor alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Alternate Names

CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IL-6R alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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STAT3 regulated ARF expression suppresses prostate cancer metastasis
Authors: Jan Pencik, Michaela Schlederer, Wolfgang Gruber, Christine Unger, Steven M. Walker, Athena Chalaris et al.
Nature Communications
Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
Blood, 2007-11-07;111(3):1021-8.
Species: Mouse
Sample Types: Air Pouch Exudate
Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood, 2007-06-13;110(6):1748-55.
Species: Mouse
Sample Types: Lavage Fluid
Applications: ELISA Development
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005-07-01;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development