Mouse Integrin alpha 11 Antibody Summary
Phe19-Pro1141
Accession # P61622
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Integrin alpha 11 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin a11 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Integrin alpha 11 in Mouse Embryonic Fibroblasts (iMEF). Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 11
Integrin alpha 11 ( ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 Integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II, plus group A Streptococcal Scl1 protein. ITGA11 is expressed by embryonic mesenchymal cells in areas that incorporate collagen during development. In adult tissue, fibroblasts express ITGA11, and a collagen:ITGA11 interaction likely contributes to the formation of myofibroblasts. Mature mouse ITGA11 is an 1166 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1119 aa extracellular domain (ECD) (aa 23-1141) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 164-345). There is one potential splice variant that shows an eight aa substitution for aa 89-119 coupled to a seven aa insertion after Gly690. Over aa 23-1141, mouse ITGA11 shares 95% and 90% aa identity with rat and human ITGA11, respectively.
Product Datasheets
Citations for Mouse Integrin alpha 11 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Generation of a novel mouse strain with fibroblast-specific expression of Cre recombinase
Authors: Jahedul Alam, Moses Musiime, Andreas Romaine, Mugdha Sawant, Arne Olav Melleby, Ning Lu et al.
Matrix Biology Plus
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Highlights of glycosylation and adhesion related genes involved in myogenesis.
Authors: Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J
BMC Genomics, 2014-07-22;15(0):621.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot
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