Mouse Integrin alpha 2/CD49b PE-conjugated Antibody

Name: Mouse Integrin alpha 2/CD49b PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1740P

Catalog #: FAB1740P Datasheet / COA / SDS

Discontinued Product

FAB1740P has been discontinued.

View all Integrin alpha 2/CD49b products.

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.

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All Integrin alpha 2/CD49b Antibodies

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Mouse Integrin alpha 2/CD49b PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed.

Source

Monoclonal Rat IgG_2B Clone # 235033

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
Tyr27-Thr1129
Accession # Q62469

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB8319A) and either (A) Rat Anti-Mouse Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1740P) or (B) Rat IgG_2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 2/CD49b

The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with beta 1 Integrin. The alpha 2 beta 1 Integrin is a collagen receptor that binds expressing cells to collagen and regulates collagen and collagenase secretion. Notably, this subunit has an "I" domain that is resistant to proteolysis. Over amino acids
(aa) 27-1129, mouse Integrin alpha 2 shares 80% aa sequence identity with human Integrin alpha 2.

Entrez Gene IDs

3673 (Human); 16398 (Mouse)

Alternate Names

alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha