Mouse Integrin alpha 5/CD49e Antibody

Catalog #: MAB1754
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1754
MAB1754-SP

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin alpha 5/CD49e Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2B Clone # 235112

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e
Phe45-Asn999 (Gln68Glu)
Accession # P11688

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Integrin alpha 5/CD49e

Flow Cytometry

2.5 µg/10⁶ cells

B16‑F1 mouse melanoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed L-929 mouse fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).

References

Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Kinashi, T. and T.A. Springer (1994) Blood Cells 20:25.
Halvorson, M.J. et al. (1995) J. Immunol. 55:4567.
Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Alternate Names

CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Integrin alpha 5/CD49e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

14-3-3theta facilitates plasma membrane delivery and function of mechanosensitive connexin 43 hemichannels.
Authors: Batra N, Riquelme M, Burra S, Jiang J
J Cell Sci, 2013-10-25;127(0):137-46.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot