Mouse Integrin  alpha 7 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3518V-025UG
FAB3518V-100UG
Detection of Integrin  alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry.
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Mouse Integrin  alpha 7 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.
Source
Monoclonal Rat IgG2B Clone # 334908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  alpha 7
Phe34-Pro1036
Accession # NP_032424
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a7 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with 0.5 µg Rat Anti-Mouse Integrin a7 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB3518V, filled histogram) or isotype control antibody (Catalog # IC013V, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 7

Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin  beta 1 to bind Laminin 1.

Entrez Gene IDs
3679 (Human); 16404 (Mouse); 81008 (Rat); 102130741 (Cynomolgus Monkey)
Alternate Names
FLJ25220; integrin alpha 7 chain; Integrin alpha 7; integrin alpha-7; integrin, alpha 7; ITGA7

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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