Mouse Integrin alpha 9 PE-conjugated Antibody

Catalog #: FAB3827P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3827P

Detection of Integrin alpha 9 in D3 Mouse Cell Line by Flow Cytometry.

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Mouse Integrin alpha 9 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Integrin alpha 1, alpha 8, alpha 9, alpha L, recombinant mouse Integrin 3, 4, 5, E, and M is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integin alpha 9
Try31-Val979
Accession # NP_598482

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Integrin a9 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3827P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 9

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C, VEGF-D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg566-Val567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively.

Entrez Gene IDs

3680 (Human); 104099 (Mouse); 685004 (Rat)

Alternate Names

ALPHA-RLC; Integrin alpha 9; integrin alpha-9; Integrin alpha-RLC; integrin, alpha 9; ITGA4L; ITGA9; RLC; RLC-alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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