Home / Integrin alpha E/CD103 / Mouse Integrin  alpha E/CD103 Antibody

Mouse Integrin  alpha E/CD103 Antibody

Catalog #: AF1990
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1990
AF1990-SP
Detection of Integrin  alpha E/CD103 in Mouse splenocytes by Flow Cytometry
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Citations (7)
FAQs
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin  alpha E/CD103 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
Phe20-Arg1113
Accession # Q60677
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha E/CD103
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Integrin  alpha E/CD103 in Mouse splenocytes by Flow Cytometry Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse Integrin  alpha E/CD103 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1990) or (B) isotype control antibody (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha E/CD103

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin  beta 7. Integrin  alpha E/ beta 7 is a receptor for E-Cadherin. Integrin  alpha E is expressed in intra‑epithelial lymphocytes and mediates adhesion of intra‑epithelial lymphocytes to epithelial cell monolayers.

Entrez Gene IDs
3682 (Human); 16407 (Mouse); 83577 (Rat)
Alternate Names
antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen

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Citations for Mouse Integrin  alpha E/CD103 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Therapeutic KRAS G12C inhibition drives effective interferon-mediated antitumor immunity in immunogenic lung cancers
    Authors: Edurne Mugarza, Febe van Maldegem, Jesse Boumelha, Christopher Moore, Sareena Rana, Miriam Llorian Sopena et al.
    Science Advances
  2. A liver immune rheostat regulates CD8 T cell immunity in chronic HBV infection
    Authors: Bosch, M;Kallin, N;Donakonda, S;Zhang, JD;Wintersteller, H;Hegenbarth, S;Heim, K;Ramirez, C;Fürst, A;Lattouf, EI;Feuerherd, M;Chattopadhyay, S;Kumpesa, N;Griesser, V;Hoflack, JC;Siebourg-Polster, J;Mogler, C;Swadling, L;Pallett, LJ;Meiser, P;Manske, K;de Almeida, GP;Kosinska, AD;Sandu, I;Schneider, A;Steinbacher, V;Teng, Y;Schnabel, J;Theis, F;Gehring, AJ;Boonstra, A;Janssen, HLA;Vandenbosch, M;Cuypers, E;Öllinger, R;Engleitner, T;Rad, R;Steiger, K;Oxenius, A;Lo, WL;Klepsch, V;Baier, G;Holzmann, B;Maini, MK;Heeren, R;Murray, PJ;Thimme, R;Herrmann, C;Protzer, U;Böttcher, JP;Zehn, D;Wohlleber, D;Lauer, GM;Hofmann, M;Luangsay, S;Knolle, PA;
    Nature
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods
    Authors: F Bayerl, DA Bejarano, G Bertacchi, AC Doffin, E Gobbini, M Hubert, L Li, P Meiser, AM Pedde, W Posch, L Rupp, A Schlitzer, M Schmitz, BU Schraml, S Uderhardt, J Valladeau-, D Wilflingse, V Zaderer, JP Böttcher
    Oncogene, 2023-01-09;0(0):e2249923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  6. Macrophages Maintain Epithelium Integrity by Limiting Fungal Product Absorption
    Authors: Aleksandra S. Chikina, Francesca Nadalin, Mathieu Maurin, Mabel San-Roman, Thibault Thomas-Bonafos, Xin V. Li et al.
    Cell
  7. Epithelial colonization by gut dendritic cells promotes their functional diversification
    Authors: Claudia A. Rivera, Violaine Randrian, Wilfrid Richer, Yohan Gerber-Ferder, Maria-Graciela Delgado, Aleksandra S. Chikina et al.
    Immunity

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Mouse Integrin  alpha E/CD103 Antibody
By Efstathios Stamatiades on 07/09/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult small intestine Species: Mouse

Cryosections from PLP immersion fixed tissue were stained for Itgae (1:100) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 (green), Itgae (red), Hoechst (blue).

Immunocytochemistry/Immunofluorescence Mouse Integrin  alpha E/CD103 Antibody AF1990