Mouse Integrin alpha E/CD103 APC-conjugated Antibody

Name: Mouse Integrin alpha E/CD103 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB1990A

Catalog #: FAB1990A Datasheet / COA / SDS

Discontinued Product

FAB1990A has been discontinued.

View all Integrin alpha E/CD103 products.

Detection of Integrin alpha E/CD103 in Mouse CD8+ Splenocytes by Flow Cytometry.

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All Integrin alpha E/CD103 Antibodies

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Mouse Integrin alpha E/CD103 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 3, rmIntegrin alpha 4, recombinant human (rh) Integrin alpha M, and rhIntegrin alpha V is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
Phe20-Arg1113 (Ser453Gly)
Accession # ABD49099

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha E/CD103 in Mouse CD8+ Splenocytes by Flow Cytometry. Mouse CD8+ splenocytes were stained with Goat Anti-Mouse Integrin aE/CD103 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1990A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha E/CD103

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin beta 7. Integrin alpha E/ beta 7 is a receptor for E-Cadherin. Integrin alpha E is expressed in intra‑epithelial lymphocytes and mediates adhesion of intra‑epithelial lymphocytes to epithelial cell monolayers.

Entrez Gene IDs

3682 (Human); 16407 (Mouse); 83577 (Rat)

Alternate Names

antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen