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Mouse Integrin  beta 6 Antibody

Catalog #: MAB2389 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2389
MAB2389-SP
Integrin beta 6 in XB2 Mouse Cell Line.
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Mouse Integrin  beta 6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 6 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 268622
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 6
Gly22-Asn706
Accession # Q9Z0T9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Integrin  beta 6 under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin beta 6 antibody in XB2 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 6 in XB2 Mouse Cell Line. Integrin beta 6 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Rat Anti-Mouse Integrin beta 6 Monoclonal Antibody (Catalog # MAB2389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta 6 is a type I transmembrane Integrin beta subunit that heterodimerizes with alpha V subunits in the epithelial restricted integrin alpha V/ beta 6. The extracellular domain of mouse Integrin beta 6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. Mouse Integrin beta 6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively.

Entrez Gene IDs
3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)
Alternate Names
Integrin beta 6; integrin beta-6; integrin, beta 6; ITGB6

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