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Mouse Integrin  beta 7 Antibody

Catalog #: MAB30601 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB30601
MAB30601-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin  beta 7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 7 extracellular domain in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 332408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 7
Glu20-His722
Accession # P26011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Adhesion Activation
The adhesion of TK-1 mouse T cell lymphoma cells (5 x 104 cells/well, treated with 1 mM MnCl2) to immobilized Recombinant Mouse MAdCAM-1 Fc Chimera (Catalog # 993-MC, 1 µg/well, 100 µL/well) was maximally enhanced (2‑2.5 fold) by 10 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Integrin beta 7 associates with the integrin alpha 4 or alpha E subunits to form heterodimers to interact with fibronectin, VCAM, MAdCAM or E-Cadherin. It is highly expressed in leukocytes and is involved in localization of leukocytes at mucosal sites.

Entrez Gene IDs
3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)
Alternate Names
Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7

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