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Mouse Integrin  beta 7 Antibody

Catalog #: AF3060 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3060
AF3060-SP
Product Details
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin  beta 7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin  beta 1, rmIntegrin beta 2, recombinant human Integrin beta 3, and rmIntegrin beta 6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 7
Glu20-His722
Accession # P26011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  beta 7
Flow Cytometry
2.5 µg/106 cells
Mouse lymph node single‑cell suspension
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Integrin beta 7 is a 130 kDa type I transmembrane glycoprotein that belongs to the integrin beta chain family. It heterodimerizes with either Integrin alpha 4 or alpha E. Whereas, Integrin alpha E beta 7 is a receptor for E-Cadherin, Integrin alpha 4/ beta 7 is a receptor for fibronectin, MAdCAM 1 and VCAM1. The extracellular domain of mouse Integrin beta 7 shares 94% and 87% amino acid sequence identity with that of rat and human Integrin beta 7, respectively.

Entrez Gene IDs
3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)
Alternate Names
Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7

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