Mouse Intelectin-1/Omentin Antibody

Catalog #: MAB8074
3 Citations Datasheet / COA / SDS

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MAB8074
MAB8074-SP

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Detection of Mouse Intelectin‑1/Omentin by Western Blot.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Intelectin-1/Omentin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Intelectin‑1/Omentin in ELISA. In direct ELISAs, no significant cross-reactivity with recombinant mouse Intelectin-2 and recombinant human Intelectin-1 is observed.

Source

Monoclonal Rat IgG_2A Clone # 746420

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Intelectin‑1/Omentin
Ala20-Ser298
Accession # O88310

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Intelectin‑1/Omentin by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Intelectin‑1/Omentin in Mouse Intestine. Intelectin-1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-1/Omentin

Intelectin (endothelial lectin; also ITLN-1 and omentin) is a 34 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed in intestinal and airway epithelium and appears to participate in insulin signaling and microbe recognition. Intelectin-1 is upregulated in asthma and contributes to allergic airway inflammation. The mouse Intelectin-1 preproprecursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature segment (aa 20-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. Soluble Intelectin-1 can be released from omental adipose tissue and malignant pleural mesothelioma. One fibrinogen-related domain exists in the mature molecule (aa 32-251). Human Intelectin-1 exists as a disulfide-linked glycosylated homotrimer; mouse is unglycosylated and a monomer. Mature mouse Intelectin-1 is 82% aa identical to human Intelectin-1.

Entrez Gene IDs

55600 (Human); 16429 (Mouse)

Alternate Names

Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Intelectin-1/Omentin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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R-spondin-3 induces secretory, antimicrobial Lgr5+ cells in the stomach
Authors: M Sigal, MDM Reinés, S Müllerke, C Fischer, M Kapalczyns, H Berger, ERM Bakker, HJ Mollenkopf, ME Rothenberg, B Wiedenmann, S Sauer, TF Meyer
Nat. Cell Biol., 2019-06-24;21(7):812-823.
Species: Bacteria - Helicobacter pylori, Mouse, Transgenic Mouse
Sample Types: Cell Culture Supernates, Whole Tissue
Applications: Immunohistochemistry, Western Blot
Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl–l-Cysteine-Sensitive Factor(s)
Authors: Satomi Kagota, Kana Maruyama-Fumoto, Saki Iwata, Miho Shimari, Shiori Koyanagi, Yayoi Shiokawa et al.
International Journal of Molecular Sciences
Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines
Authors: SS Rao, Y Hu, PL Xie, J Cao, ZX Wang, JH Liu, H Yin, J Huang, YJ Tan, J Luo, MJ Luo, SY Tang, TH Chen, LQ Yuan, EY Liao, R Xu, ZZ Liu, CY Chen, H Xie
Bone Res, 2018-03-30;6(0):9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot