Mouse Intelectin-1/Omentin Antibody

Detection of Mouse Intelectin‑1/Omentin by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intelectin‑1/Omentin in Mouse Intestine. Intelectin-1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Intelectin-1/Omentin by Immunohistochemistry Microbiota suppresses transcriptional nutrient stress response and activates mTOR signaling. A) Overlay of nutrient starvation stress response transcription factor genes upon gene expression volcano plot of all GF and SPF epithelial cells. B) Violin plot of Atf3 and Jun expression across GF and SPF epithelial cells. C) Violin plot of Fos expression across GF and SPF epithelial cells and quantification and representative image of Fos in situ hybridization within GF and SPF jejunal/ileal small intestinal crypts. D) Violin plots and E) UMAP visualization of Rps6kb1 and Rps6 epithelial subsets in GF and SPF epithelial cells. *, p < .01 comparing GF and SPF clusters by Wilcoxon rank sum test and >0.75 Log2 fold change. F) pS6 staining and quantification of jejunal/ileal small intestinal crypts from GF and SPF mice. G) Immunofluorescence co-staining of BrdU, pS6, and Itln1 in GF and SPF jejunal/ileal small intestinal crypts. Arrows indicate co-expression of Itln1 and pS6. Representative images shown (n = 4–6). Each point represents the mean quantification 20 crypts from one mouse (n = 4–6). Stains were quantified using CellProfiler. Bars represent mean ± SEM. **, p < .005 by two-tailed Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35939622), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Intelectin-1/Omentin by Immunohistochemistry Microbiota suppresses transcriptional nutrient stress response and activates mTOR signaling. A) Overlay of nutrient starvation stress response transcription factor genes upon gene expression volcano plot of all GF and SPF epithelial cells. B) Violin plot of Atf3 and Jun expression across GF and SPF epithelial cells. C) Violin plot of Fos expression across GF and SPF epithelial cells and quantification and representative image of Fos in situ hybridization within GF and SPF jejunal/ileal small intestinal crypts. D) Violin plots and E) UMAP visualization of Rps6kb1 and Rps6 epithelial subsets in GF and SPF epithelial cells. *, p < .01 comparing GF and SPF clusters by Wilcoxon rank sum test and >0.75 Log2 fold change. F) pS6 staining and quantification of jejunal/ileal small intestinal crypts from GF and SPF mice. G) Immunofluorescence co-staining of BrdU, pS6, and Itln1 in GF and SPF jejunal/ileal small intestinal crypts. Arrows indicate co-expression of Itln1 and pS6. Representative images shown (n = 4–6). Each point represents the mean quantification 20 crypts from one mouse (n = 4–6). Stains were quantified using CellProfiler. Bars represent mean ± SEM. **, p < .005 by two-tailed Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35939622), licensed under a CC-BY license. Not internally tested by R&D Systems.